Is defibrotide's activity on leukocytes adenosine-receptor mediated? An "in vitro"--"ex vivo" appraisal

Thrombosis Research
P AlbericoL Ferro

Abstract

Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, suggesting that PGI2 is not involved in DFT's activity, at least under our experimental conditions. Non-PMN leukocytes behaved differently "ex vivo" than PMNs. DFT's activity was not blunted by 8-phenyltheophylline, suggesting that DFT acts on this kind of cell through a different mechanism. The "in vitro" experiment did not confirm "ex vivo" studies suggesting that in the "in vitro" experiment "something" is missing that is present in "ex vivo" experiments.

References

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Citations

Feb 21, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Constantine S MitsiadesPaul G Richardson

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