Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy?

Malaria Journal
T HänscheidM P Grobusch

Abstract

Detection of malaria pigment (or haemozoin; Hz)-containing leukocytes may have prognostic relevance in malaria; however, studies reported conflicting results, with microscopic counts suggestive of being inaccurate and imprecise. Numbers of Hz-containing leukocytes from a malaria patient obtained with a flow cytometer counting 50.000 gated events were compared with thin film microscopy as applied under field conditions. Flow cytometry identified 5.8% Hz-containing monocytes and 1.8% Hz-containing neutrophils. The microscopic examination yielded 10% and 13% of Hz-containing monocytes, as well as 0% and 0.5% of Hz-containing neutrophils for observers one and two, respectively. Novel, robust and affordable cytometric methods should be evaluated in the field as they may assist in utilizing Hz-containing cells as clinically useful parameter.

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Citations

Aug 14, 2013·Trends in Parasitology·Márcia BouraThomas Hänscheid
Nov 1, 2012·Malaria Journal·Matthias WillmannJanet Cox-Singh
Dec 2, 2010·Malaria Journal·Germán Campuzano-ZuluagaMartin P Grobusch
Apr 23, 2014·Tropical Parasitology·Sarita MohapatraRajni Gaind
Mar 27, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Maria RebeloThomas Hänscheid
Mar 5, 2010·American Journal of Hematology·Anuraj H Shankar, Wafaie W Fawzi
Apr 17, 2015·Malaria Journal·Maria RebeloThomas Hänscheid
May 9, 2012·Malaria Research and Treatment·Rosangela FritaThomas Hänscheid
Dec 18, 2012·Expert Review of Anti-infective Therapy·Andrea L ConroyKevin C Kain

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Methods Mentioned

BETA
polarizing microscopy
flow cytometry
light microscopy

Software Mentioned

Dyn ®
Dyn
Cell

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