Is glandular formation of nitric oxide a prerequisite for muscarinic secretion of fructose in the guinea-pig seminal vesicle?

Urological Research
I EhrenN P Wiklund

Abstract

The significance of nitric oxide (NO) formation in seminal secretion was studied in guinea-pig seminal vesicles. The nitric oxide synthase (NOS) activity was estimated and reduced nicotinamide-adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry was performed. Furthermore, cyclic guanosine 3,5-monophosphate (cGMP) concentration as well as fructose secretion from isolated vesicles was estimated. High Ca2+-dependent NOS activity as well as prominent glandular NADPH-diaphorase staining was found in the secretory epithelium. The NOS inhibitors N(G)-nitro L-arginine methyl ester (L-NAME) and N(G)-nitro L-arginine (L-NNA) inhibited carbachol-induced fructose secretion but the D-isomer to L-NAME had no effect. When L-arginine was administered together with L-NAME, no inhibitory effect on the carbachol-induced fructose secretion could be seen. Nerve-induced fructose secretion was also inhibited by L-NAME. The NO donor glyceryl trinitrate (GTN) increased the fructose secretion. Carbachol or GTN did not increase cGMP levels, nor was fructose secretion inhibited by a guanylate cyclase inhibitor (ODQ). Our results suggests that glandular NO production is a prerequisite for muscarinic fructose secretion in the seminal vesicle vi...Continue Reading

References

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Citations

Aug 21, 2009·Journal of Molecular Neuroscience : MN·Maria Christina W AvellarCatarina S Porto
Jun 1, 2005·Life Sciences·Elisabeth MarósticaCatarina S Porto
Oct 20, 2005·Acta Obstetricia Et Gynecologica Scandinavica·B MörlinM Hammarström
Feb 17, 2006·Molecular and Cellular Endocrinology·Margarete HamamuraCatarina S Porto
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Maria Christina W AvellarCatarina S Porto
Oct 19, 2004·Acta Obstetricia Et Gynecologica Scandinavica·Birgitta MörlinNils-Olof Sjöstrand
Aug 16, 2005·Acta Obstetricia Et Gynecologica Scandinavica·Birgitta Mörlin, Margareta Hammarström
Nov 27, 2009·Systems Biology in Reproductive Medicine·Nader SalamaSusumu Kagawa

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