Is His54 a gating residue for the ferritin ferroxidase site?

Biochimica Et Biophysica Acta
Caterina BernacchioniPaola Turano

Abstract

Ferritin is a ubiquitous iron concentrating nanocage protein that functions through the enzymatic oxidation of ferrous iron and the reversible synthesis of a caged ferric-oxo biomineral. Among vertebrate ferritins, the bullfrog M homopolymer ferritin is a frequent model for analyzing the role of specific amino acids in the enzymatic reaction and translocation of iron species within the protein cage. X-ray crystal structures of ferritin in the presence of metal ions have revealed His54 binding to iron(II) and other divalent cations, with its imidazole ring proposed as "gate" that influences iron movement to/from the active site. To investigate its role, His54 was mutated to Ala. The H54A ferritin variant was expressed and its reactivity studied via UV-vis stopped-flow kinetics. The H54A variant exhibited a 20% increase in the initial reaction rate of formation of ferric products with 2 or 4 Fe²⁺/subunit and higher than 200% with 20 Fe²⁺/subunit. The possible meaning of the increased efficiency of the ferritin reaction induced by this mutation is proposed taking advantage of the comparative sequence analysis of other ferritins. The data here reported are consistent with a role for His54 as a metal ion trap that maintains the corr...Continue Reading

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Sep 4, 2015·Acta Crystallographica. Section D, Biological Crystallography·Cecilia PozziStefano Mangani
Jun 14, 2015·Biochimica Et Biophysica Acta·Andrea Mozzarelli, Loredano Pollegioni
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Aug 4, 2021·Chemistry : a European Journal·Silvia CiambellottiCecilia Pozzi

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