Is hypothermia a stress condition in HepG2 cells? Expression and localization of Hsp70 in human hepatoma cell line

Tissue & Cell
Alegna RadaMirian Strauss


To understand hypothermia as a stress condition we determined the expression and localization of Hsp70 under hyperthermic and hypothermic stress in human hepatoma HepG2 cells. Western blot analysis indicates that there was a statistically significant increase of Hsp70 expression under thermal stresses. Immunohistochemically, the distribution of inducible Hsp70 in stressed cells showed a granular pattern mostly in the cytoplasm. At subcellular level, Hsp70 was localized in the nucleus, vacuoles, cytoskeletal components and dispersed throughout the cytoplasm. Accumulation of Hsp70 in cells under hypothermia could be related to restitution of cell equilibrium modified by this thermal stress condition. The protective effect of hypothermia could be associated with promotion of Hsp expression. We suggest that hypothermia is a stress capable of inducing Hsp70 expression in human HepG2 cells.


Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J GoldsteinM Inouye
Jan 17, 1997·The Journal of Biological Chemistry·K E Cullen, K D Sarge
Nov 21, 1997·Experimental Cell Research·G MorcilloJ L Díez
May 9, 1998·Journal of Molecular and Cellular Cardiology·C Y SuC C Lai
Aug 8, 1998·The International Journal of Biochemistry & Cell Biology·T T WangY K Lai
Feb 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S EllisR L Anderson
May 2, 2000·Cryobiology·B J Griffiths, P J Evans
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·E A NollenH H Kampinga
Aug 15, 2003·World Journal of Gastroenterology : WJG·Xiao-Ling DengDa-Peng Wei
Sep 23, 2003·Shock·Lizbeth CornivelliAntonio De Maio


Oct 9, 2008·Journal of Molecular Histology·Giuseppina TurturiciGabriella Sconzo
Nov 17, 2007·Forensic Science International : Synergy·J PreussB Madea
Feb 22, 2007·Journal of Medical Virology·Arturo Cabrera-HernandezDuncan R Smith
Mar 17, 2010·Journal of Molecular Biology·Naghmeh S SarrafIrena Ekiel
Jun 25, 2019·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Katsuya KamiYoshinobu Ohira
Jul 1, 2018·International Journal of Legal Medicine·Takahiro UmeharaKazuya Ikematsu
Dec 19, 2012·International Journal of Legal Medicine·Makoto SakuradaYasuhiro Ueno

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.