Is oocyte meiotic spindle morphology associated with embryo ploidy? A prospective cohort study

Fertility and Sterility
Liza TiliaMichael G Chapman

Abstract

To explore whether an association exists between oocyte meiotic spindle morphology visualized by polarized light microscopy at the time of intracytoplasmic sperm injection and the ploidy of the resulting embryo. Prospective cohort study. Private IVF clinic. Patients undergoing preimplantation genetic screening/diagnosis (n = 113 patients). Oocyte meiotic spindles were assessed by polarized light microscopy and classified at the time of intracytoplasmic sperm injection as normal, dysmorphic, translucent, telophase, or no visible spindle. Single blastomere biopsy was performed on day 3 of culture for analysis by array comparative genomic hybridization. Spindle morphology and embryo ploidy association was evaluated by regression methods accounting for non-independence of data. The frequency of euploidy in embryos derived from oocytes with normal spindle morphology was significantly higher than all other spindle classifications combined (odds ratio [OR] 1.93, 95% confidence interval [CI] 1.33-2.79). Oocytes with translucent (OR 0.25, 95% CI 0.13-0.46) and no visible spindle morphology (OR 0.35, 95% CI 0.19-0.63) were significantly less likely to result in euploid embryos when compared with oocytes with normal spindle morphology. Th...Continue Reading

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Citations

Jul 10, 2018·Endocrine Reviews·Ali AbbaraWaljit S Dhillo
Jul 14, 2017·Journal of Ovarian Research·Ana Maria SanchezPaola Viganò
Jan 13, 2019·Journal of Assisted Reproduction and Genetics·Zuzana HolubcováAleš Hampl
Jul 18, 2018·Reproductive Medicine and Biology·Hiroyuki TomariToshitaka Horiuchi

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