Is platelet-activating factor produced during hemodialysis with AN-69 polyacrylonitrile membrane?

Nephron
C IatrouS Antonopoulou

Abstract

Platelet-activating factor (PAF) production during hemodialysis (HD) with cuprophane (CU) membrane has previously been demonstrated, while the results regarding PAF production during HD with AN-69 polyacrylonitrile membrane are dubious. In this study an attempt is made to show that PAF is produced during HD using AN-69 membrane while comparing this production with the corresponding one from HD with CU. Since previous studies have indicated that PAF, like the complement system, could also be implicated in HD-related leukopenia and thrombocytopenia (especially when CU membrane is used), the circulating leukocyte and platelet counts as well as the C3a-desArg and SC5b-9 (soluble, nonlytic form of the terminal complement complex) levels were measured. Ten hemodialyzed patients were subjected to HD with CU and AN-69 membranes for 2 consecutive weeks (first week with CU and second with AN-69). During the third HD session of each week and at different times (0, 2, 5, 15, 30, 60, 180 and 240 min), the PAF levels in the blood as well as the leukocyte and platelet counts were measured, while the circulating levels of the C3a-desArg and SC5b-9 were measured at 0, 5, 15, 60 and 240 min. PAF was detected by ethanol extraction, followed by pu...Continue Reading

Citations

May 18, 2012·Kidney International·John T Daugirdas, Angelito A Bernardo

Related Concepts

Cuprammonium cellulose
AN-69 Sodium
Acrylic Resins
Acrylonitrile
Alphacel
Complement Activation
Hemodialysis
Differential White Blood Cell Count Procedure
Membranes, Artificial
Platelet Activating Factor

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