PMID: 8614034Apr 1, 1996Paper

Is sepsis-induced apoptosis associated with macrophage dysfunction?

The Journal of Trauma
Alfred AyalaI H Chaudry

Abstract

Apoptosis (A O) is a pathological process by which cells undergo a form of inducible nonnecrotic cellular suicide. In vitro studies suggest that changes in the rate of macrophage (Mo) A O may be associated with elevated proinflammatory cytokine secretory capacity, such as interleukin-1 beta (IL-1 beta) (via IL-1 converting enzyme activation). Furthermore, it has been reported that Mo are activated during early (0-4 hours) experimental septic insult to act as sources of proinflammatory cytokines, such as IL-1. However, with the progression of sepsis, these same cells become refractory to further stimulation (appearing dysfunctional). Nonetheless, it remains unknown if this acquired immunosuppression (dysfunction) is associated with an acceleration in macrophage A O. To determine this, male C3H/HeN mice were subjected to sepsis (cecal ligation and puncture, CLP) or sham-CLP and 4 or 24 hours thereafter Mo were isolated from the peritoneum (PMo) and liver (KMo). Macrophage monolayers were lysed either after stimulation with lipopolysaccharide (LPS) (10 microgram/mL, 24 hours) in vitro or immediately (ex vivo) before LPS stimulation and the cytoplasmic cell fraction was retained. The extent of A O was determined using a cell-death ...Continue Reading

References

Jan 1, 1992·Annual Review of Immunology·J J CohenK S Sellins
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K A HogquistD D Chaplin
Mar 15, 1993·Biochemical and Biophysical Research Communications·M SarihA Adam
Feb 1, 1995·Critical Care Medicine·S L Barriere, S F Lowry
Dec 1, 1993·Immunology Today·L M Schwartz, B A Osborne

❮ Previous
Next ❯

Citations

Mar 14, 2002·Critical Care Medicine·John A. Baugh, Richard Bucala
Dec 7, 2011·Infection and Immunity·Laura BrudeckiMohamed El Gazzar
Sep 10, 2003·Journal of Leukocyte Biology·P S GrutkoskiA Ayala
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert A MitchellRichard Bucala
Dec 17, 1997·Scandinavian Journal of Gastroenterology·R AnderssonV Soltesz
Jun 23, 2007·The Journal of Surgical Research·Mareike LefflerMarc G Jeschke
Jul 5, 2007·Immunology·Anna SinistroAlberto Bergamini
Apr 1, 1997·The Journal of Surgical Research·A AyalaI H Chaudry
Aug 12, 1998·The Journal of Surgical Research·A AyalaI H Chaudry
Aug 26, 1998·Biochemical and Biophysical Research Communications·J BattleD Williams
Nov 25, 2000·The Journal of International Medical Research·K TammeR Talvik
Apr 9, 2005·Journal of Leukocyte Biology·Doreen E WescheAlfred Ayala
Mar 27, 2007·Annals of the New York Academy of Sciences·Helle Evi SimovartAade Liigant
Oct 24, 2008·Clinical and Vaccine Immunology : CVI·Anna SinistroAlberto Bergamini
Aug 27, 2005·American Journal of Physiology. Cell Physiology·Y OsawaC W Emala
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caroline K HuAlfred Ayala
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Makiko KobayashiFujio Suzuki
Jul 5, 2003·Scandinavian Journal of Surgery : SJS : Official Organ for the Finnish Surgical Society and the Scandinavian Surgical Society·H TapferR Talvik
Feb 11, 2020·Frontiers in Physiology·Josiane Fernandes SilvaRita C Tostes
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Asit K DeCarol L Miller-Graziano
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcin F OsuchowskiDaniel G Remick
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcin F OsuchowskiDaniel G Remick

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis