Is the cytoplasmic loop of MerT, the mercuric ion transport protein, involved in mercury transfer to the mercuric reductase?

FEBS Letters
Emmanuel RossyJacques Covès

Abstract

In MerT, the mercury transporter, a first cysteine pair, located in the first trans-membrane helix, receives mercury from the periplasm. Then, a second cysteine pair, housed in a cytoplasmic loop connecting the second and the third trans-membrane helices, is thought to transfer the metal to another cysteine pair located in the N-terminal extension of the mercuric reductase. We found that a 23-amino acid synthetic peptide corresponding to the cytoplasmic loop can bind one mercury atom per molecule and that this mercury atom can be transferred specifically to MerAa. The solution structure of Hg-bound ppMerT has been solved by 1H NMR spectroscopy.

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Dec 1, 1992·Molecular & General Genetics : MGG·R Sedlmeier, J Altenbuchner
Apr 1, 1992·European Journal of Biochemistry·L Sahlman, B H Jonsson
Jun 1, 2002·Current Opinion in Chemical Biology·Stanley J OpellaGianluigi Veglia
Jul 13, 2002·Biopolymers·Tara M DeSilvaStanley J Opella
Aug 28, 2002·Biochemical Society Transactions·N L BrownJ R Wilson
Jun 28, 2003·FEMS Microbiology Reviews·Nigel L BrownJon L Hobman
Jun 28, 2003·FEMS Microbiology Reviews·Tamar BarkayAnne O Summers
Nov 19, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Emmanuel RossyJacques Covès

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Citations

Jul 10, 2012·Applied and Environmental Microbiology·Zachary FreedmanTamar Barkay
Apr 26, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mathieu SchueNigel L Brown
Dec 8, 2014·Protein Expression and Purification·Kien LyAlok K Mitra
Feb 7, 2017·Chemistry : a European Journal·Matthieu StarckPascale Delangle
Nov 30, 2018·Metallomics : Integrated Biometal Science·Ramesh KarriGouriprasanna Roy
Dec 15, 2017·Environmental Science and Pollution Research International·Preeti Ranawat, Seema Rawat
Jan 29, 2019·Frontiers in Microbiology·Ling-Dong ShiHe-Ping Zhao
Oct 18, 2014·Chemistry : a European Journal·Colette LebrunPascale Delangle
Feb 2, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Agnieszka NiedźwieckaPascale Delangle
Dec 16, 2010·Journal of the American Chemical Society·Anaïs M PujolPascale Delangle
Jul 14, 2007·Science·James G Omichinski

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