Is the gene encoding Chibby implicated as a tumour suppressor in colorectal cancer ?

BMC Cancer
Sophie GadPierre Laurent-Puig

Abstract

A novel member of the Wnt signalling pathway, Chibby, was recently identified. This protein inhibits Wnt/beta-catenin mediated transcriptional activation by competing with Lef-1 (the transcription factor and target of beta-catenin) to bind to beta-catenin. This suggests that Chibby could be a tumour suppressor protein. The C22orf2 gene coding Chibby is located on chromosome 22, a region recurrently lost in colorectal cancer. Activation of the Wnt pathway is a major feature of colorectal cancer and occurs through inactivation of APC or activation of beta-catenin. All of this led us to analyse the possible implication of Chibby in colorectal carcinogenesis. First, 36 tumour and matched normal colonic mucosa DNA were genotyped with five microsatellite markers located on chromosome 22 to search for loss of heterozygosity. Then, mutation screening of the C22orf2 coding sequence and splice sites was performed in the 36 tumour DNA. Finally, expression of Chibby was analysed by quantitative RT-PCR on 10 patients, 4 with loss of heterozygosity (LOH) on chromosome 22. Loss of heterozygosity involving the C22orf2 region was detected in 11 out of 36 patients (30%). Sequencing analysis revealed a known variant, rs3747174, in exon 5: T321C l...Continue Reading

References

Apr 20, 2000·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·P Laurent-PuigP H Cugnenc
Jun 1, 2002·Science·Randall T MoonNorbert Perrimon

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Citations

Jan 21, 2017·Cell Cycle·Victoria FischerKen-Ichi Takemaru
Oct 15, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hong-Tao XuEn-Hua Wang
Mar 30, 2006·World Journal of Gastroenterology : WJG·Marion M SchuiererAnja-Katrin Bosserhoff
Jan 26, 2010·Experimental Cell Research·Karl VandepoeleFrans van Roy

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Methods Mentioned

BETA
electrophoresis
PCR
genotyping

Software Mentioned

SDS
Genotyper
[UNK]

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