Is the in vitro ejection of bacteriophage DNA quasistatic? A bulk to single virus study.

Biophysical Journal
N ChiaruttiniV Viasnoff

Abstract

Bacteriophage T5 DNA ejection is a complex process that occurs on several timescales in vitro. By using a combination of bulk and single phage measurements, we quantitatively study the three steps of the ejection-binding to the host receptor, channel-opening, and DNA release. Each step is separately addressed and its kinetics parameters evaluated. We reconstruct the bulk kinetics from the distribution of single phage events by following individual DNA molecules with unprecedented time resolution. We show that, at the single phage level, the ejection kinetics of the DNA happens by rapid transient bursts that are not correlated to any genome sequence defects. We speculate that these transient pauses are due to local phase transitions of the DNA inside the capsid. We predict that such pauses should be seen for other phages with similar DNA packing ratios.

References

Mar 1, 1978·Biopolymers·S C Riemer, V A Bloomfield
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S H Northrup, H P Erickson
Jul 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Alex EvilevitchWilliam M Gelbart
Nov 16, 2004·Biophysical Journal·Marta de FrutosEric Raspaud
Nov 24, 2004·Biophysical Journal·Prashant K PurohitRob Phillips
Mar 9, 2005·Current Biology : CB·Stéphanie MangenotLucienne Letellier
Aug 4, 2005·The European Physical Journal. E, Soft Matter·M de FrutosE Raspaud
May 9, 2006·Biophysical Journal·Mandar M InamdarRob Phillips
Mar 6, 2007·Journal of Molecular Biology·David LöfAlex Evilevitch
Jul 6, 2007·The EMBO Journal·Celia PlissonElena V Orlova
Sep 1, 2007·The European Physical Journal. E, Soft Matter·M Castelnovo, A Evilevitch
Sep 25, 2007·Biophysical Journal·Alex EvilevitchCharles M Knobler
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Derek N FullerDouglas E Smith
Oct 14, 2008·Journal of Molecular Biology·A LeforestierF Livolant
Mar 5, 2009·Physical Review Letters·Richard MatthewsJ M Yeomans
Dec 1, 2009·Journal of Molecular Biology·Amélie Leforestier, Françoise Livolant
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Davide MarenduzzoCristian Micheletti

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Citations

Jul 19, 2013·Journal of Biological Physics·Amélie Leforestier
Jul 19, 2013·Journal of Biological Physics·J P MahalikM Muthukumar
Sep 15, 2011·The Journal of Chemical Physics·I Ali, D Marenduzzo
Oct 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ting LiuAlex Evilevitch
Nov 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Davide MarenduzzoDe Witt Sumners
Mar 29, 2014·Molecular Microbiology·Kristin N ParentSherwood R Casjens
Jul 26, 2011·Current Opinion in Microbiology·Aurélie BertinLucienne Letellier
Jun 21, 2015·Nucleic Acids Research·Dong LiAlex Evilevitch
May 10, 2015·The Journal of Chemical Physics·James M Polson
Jun 26, 2012·Current Biology : CB·David Van ValenRob Phillips
Apr 12, 2016·The Journal of Physical Chemistry. B·Aaron M GoldfainVinothan N Manoharan
May 7, 2016·The Journal of Physical Chemistry. B·Marta de FrutosFrançoise Livolant
May 14, 2016·The Journal of Physical Chemistry. B·Krista G FreemanAlex Evilevitch
Feb 7, 2013·Nature Reviews. Microbiology·Ian J Molineux, Debabrata Panja
Jun 14, 2019·The Journal of Biological Chemistry·Nina K BroekerStefanie Barbirz
Mar 19, 2013·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Serge G LemayIan J Molineux
Feb 24, 2015·The Journal of Biological Chemistry·Verónica A González-GarcíaJosé L Carrascosa
Oct 24, 2019·Physical Review. E·Qin LiangJeff Z Y Chen
Sep 7, 2019·Physical Review Letters·Tine CurkRudolf Podgornik
May 31, 2018·Viruses·Nina K BroekerStefanie Barbirz
Oct 20, 2020·Frontiers in Microbiology·Mareike S StephanStefanie Barbirz
Aug 18, 2010·Biophysical Journal·David WuRob Phillips

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