Is the isotopic composition of nitrous oxide an indicator for its origin from nitrification or denitrification? A theoretical approach from referred data and microbiological and enzyme kinetic aspects

Rapid Communications in Mass Spectrometry : RCM
Hanns-Ludwig SchmidtReinhard Well

Abstract

Literature data on the isotopic composition of nitrous oxide indicate a general predominance of the alpha-15N-isotopomer and a parallel 18O-enrichment in N2O from nitrification and denitrification, respectively. As the kinetic isotope effects on any individual reactions of the two processes lead to depletions of the heavy isotopes of nitrogen and oxygen in the products, the observed enrichments could mainly be caused by enzymatic reduction of NO, provided it occurs via a symmetric intermediate like hyponitrite; infrared data are in favour of large differences between the binding constants of the isotopomers and isotopologues of this compound. As a matter of fact one of the mechanisms discussed for the nitric oxide reductase from certain microorganisms implies the parallel binding of two NO molecules and the formation of a symmetrical intermediate, while that of the enzyme from other microorganisms reduces NO in a sequential mechanism. In addition, isotope effects on the reduction of N2O to N2 must contribute to the observed isotope characteristics of N2O, especially in context with denitrification. Therefore, the known enzymatic reaction pathways suggest that the alpha-15N-isotopomer preference and the 18O-signature of the prod...Continue Reading

Citations

Apr 20, 2005·Rapid Communications in Mass Spectrometry : RCM·Jan Willem Van GroenigenChris van Kessel
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Aug 15, 2009·Rapid Communications in Mass Spectrometry : RCM·R Well, H Flessa
Aug 5, 2014·Rapid Communications in Mass Spectrometry : RCM·Lena RoheReinhard Well
Dec 9, 2008·Isotopes in Environmental and Health Studies·Stefanie Daniela GoldbergGerhard Gebauer
Feb 17, 2015·Environmental Science & Technology·L Camille JonesScott Fendorf
Aug 15, 2014·Environmental Science & Technology·Hui YangEric L Hegg
Nov 29, 2005·Perception·Deborah L Wells
Jun 18, 2020·Rapid Communications in Mass Spectrometry : RCM·Longfei YuJoachim Mohn
May 21, 2017·Scientific Reports·Timothy J CloughKarl G Richards
Mar 5, 2011·Environmental Science & Technology·Gayan RubasinghegeVicki H Grassian

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