PMID: 9443339Jan 27, 1998Paper

Is the molten globule a third thermodynamic state of protein? The example of alpha-lactalbumin

Proteins
W Pfeil

Abstract

Thermal and denaturant-induced transitions of the acid molten globule state of bovine alpha-lactalbumin (acid [A] state) are analyzed by scanning calorimetry, titration calorimetry, viscosimetry, and derivative spectroscopy. A denaturant-induced heat effect of the A state is shown by a calorimetric difference titration of the A-state versus unfolded (reduced) alpha-lactalbumin. However, changes of viscosity and derivative spectra do not parallel the heat effect. At thermal denaturation monitored by derivative spectroscopy and scanning microcalorimetry the presence of a gradual transition in alpha-lactalbumin A state is shown. The results are consistent with the existence of tertiary interactions in the A state and the absence of a cooperative unfolding transition of the molten globule. The results do not support the idea that the molten globule is a third thermodynamic state.

References

Sep 15, 1976·Journal of Molecular Biology·K KuwajimaS Sugai
Jul 20, 1992·Journal of Molecular Biology·G I Makhatadze, P L Privalov
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D StigterK A Dill
Oct 21, 1986·Biochemistry·K Gekko, Y Hasegawa
Jan 1, 1972·Methods in Enzymology·J M Sturtevant
Apr 1, 1981·Biophysical Chemistry·W Pfeil
Dec 28, 1981·FEBS Letters·D A DolgikhO B Ptitsyn
Oct 3, 1995·Biochemistry·P Polverino de LauretoA Fontana
Mar 14, 1994·FEBS Letters·O B Ptitsyn, V N Uversky
Jan 1, 1995·Advances in Protein Chemistry·G I Makhatadze, P L Privalov
Jan 1, 1995·Advances in Protein Chemistry·O B Ptitsyn
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K Kuwajima
May 24, 1996·Journal of Molecular Biology·P L Privalov
Jun 1, 1996·Nature Structural Biology·O Ptitsyn

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Citations

Jul 31, 1998·Protein Science : a Publication of the Protein Society·M IkeguchiS Sugai
Oct 7, 1998·Protein Science : a Publication of the Protein Society·K GastG Damaschun
Dec 14, 1999·Protein Science : a Publication of the Protein Society·P Polverino de LauretoA Fontana
Sep 9, 2008·Biochemical and Biophysical Research Communications·Tatsuro KamijimaMakoto Demura
Oct 29, 2002·Proteins·Kenji SasaharaKatsutoshi Nitta
Jan 26, 2002·Protein Engineering·T KoshibaK Nitta
Aug 6, 1999·Protein Engineering·L H GreeneK Brew
Nov 30, 2006·The Protein Journal·Masanori YasuiKatsutoshi Nitta
Oct 28, 2008·Journal of Neurosurgery·Andrej StenoJuraj Steno
Aug 19, 2007·Biochemistry·Ravindra Singh PrajapatiRaghavan Varadarajan
Mar 30, 2001·Biochimica Et Biophysica Acta·C G BrouilletteD W Borhani
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

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