Is there a preferential interaction between cholesterol and tryptophan residues in membrane proteins?

Biochemistry
Andrea HoltJ Antoinette Killian

Abstract

Recently, several indications have been found that suggest a preferential interaction between cholesterol and tryptophan residues located near the membrane-water interface. The aim of this study was to investigate by direct methods how tryptophan and cholesterol interact with each other and what the possible consequences are for membrane organization. For this purpose, we used cholesterol-containing model membranes of dimyristoylphosphatidylcholine (DMPC) in which a transmembrane model peptide with flanking tryptophans [acetyl-GWW(LA)8LWWA-amide], called WALP23, was incorporated to mimic interfacial tryptophans of membrane proteins. These model systems were studied with two complementary methods. (1) Steady-state and time-resolved Förster resonance energy transfer (FRET) experiments employing the fluorescent cholesterol analogue dehydroergosterol (DHE) in combination with a competition experiment with cholesterol were used to obtain information about the distribution of cholesterol in the bilayer in the presence of WALP23. The results were consistent with a random distribution of cholesterol which indicates that cholesterol and interfacial tryptophans are not preferentially located next to each other in these bilayer systems. (...Continue Reading

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Citations

Oct 22, 2009·European Biophysics Journal : EBJ·Luís M S LouraManuel Prieto
Dec 19, 2009·European Biophysics Journal : EBJ·Andrea Holt, J Antoinette Killian
Mar 20, 2010·European Biophysics Journal : EBJ·Luís M S LouraFábio Fernandes
Aug 14, 2009·Journal of the American Chemical Society·Sivaramakrishnan RamaduraiBert Poolman
Dec 14, 2011·The Journal of Cell Biology·Maud de Saint-JeanGuillaume Drin
Mar 10, 2016·International Journal of Biological Macromolecules·Tiago SantosC Cruz
Aug 30, 2008·Chemistry and Physics of Lipids·Rodrigo F M de AlmeidaManuel Prieto
Sep 17, 2011·Medicinal Research Reviews·Frederick HarrisDavid A Phoenix
Oct 25, 2013·Protein Science : a Publication of the Protein Society·Yuanli SongCharles R Sanders
Nov 19, 2008·Biochimica Et Biophysica Acta·Luís M S LouraManuel Prieto
Aug 20, 2009·The Journal of Physical Chemistry. B·Francesco ScarpelliMartina Huber
Jun 30, 2019·The Journal of Physical Chemistry. B·Madeleine R LandryGrace Y Stokes

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