Is there a unique melting temperature for two-state proteins?

Journal of Computational Chemistry
D K Klimov, D Thirumalai

Abstract

Thermal unfolding (or folding) in many proteins occurs in an apparent two-state manner, suggesting that only two states, unfolded and folded, are populated. At the melting temperature, Tm, the two states coexist. Using lattice models with side chains we show that individual residues become structured at temperatures that deviate from Tm, which implies that partially folded conformations make substantial contribution to thermodynamic properties of two-state proteins. We also find that the folding cooperativity for a given residue is linked to its accessible surface area. These results are consistent with the experiments on GCN4-like zipper peptide, which showed that local melting temperatures differ from Tm. Analysis of thermal unfolding of six proteins shows that deltaT/Tm approximately N(-1), where deltaT is the transition width and N is the number of residues. This scaling allows us to conclude that, when corrected for finite size effects, folding cooperativity can be captured using coarse grained models.

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Citations

Jun 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Santosh Kumar JhaJayant B Udgaonkar
Aug 24, 2010·The Journal of Biological Chemistry·Tanay M DesaiVictor Muñoz
May 14, 2009·HFSP Journal·Victor MuñozDavid de Sancho
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hairong Ma, Martin Gruebele
Oct 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jason E OllerenshawLewis E Kay
May 29, 2007·Journal of Molecular Biology·Feng Liu, Martin Gruebele
Sep 10, 2005·Biophysical Journal·Ting WangFeng Gai
Apr 14, 2012·The Journal of Physical Chemistry. B·Zhenxing LiuD Thirumalai
Jun 27, 2006·Nature·Mourad SadqiVictor Muñoz
Feb 9, 2005·Physical Review Letters·Mai Suan LiD Thirumalai
Sep 12, 2020·Accounts of Chemical Research·Luis Alberto CamposVictor Muñoz
Jan 13, 2006·The Journal of Physical Chemistry. a·Maksim KouzaD Thirumalai

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