Feb 9, 2012

Is there an optimal pH for culture media used in clinical IVF?

Human Reproduction Update
Jason E Swain

Abstract

Reducing environmental stress imposed upon gametes and embryos in the IVF laboratory is crucial in optimizing culture conditions and development. One environmental parameter of particular importance is external pH (pHe) of culture media. An optimal pHe has not been identified. Electronic searches were performed using keywords focused on pH and the embryo using PUBMED through August 2011, with no limits placed on a beginning time. Examples of keywords include CO(2), bicarbonate and hydrogen ion. Relevant papers were then examined to obtain additional publications. Determining optimal pHe is problematic due to difficulty in isolating pHe from other variables, such as CO(2) and bicarbonate. Various commercial media companies recommend differing pHe ranges, most within the range of 7.2-7.4, with some companies recommending altering pHe based on the gamete or stage of the embryo. However, changing pHe during culture has not been experimentally shown to improve outcomes. Further complicating attempts to define an optimal pHe is that media components can impact intracellular pH (pHi). As a result, media with different concentrations of substances, such as lactate or amino acids, may have different pHi, despite being in the same pHe. D...Continue Reading

  • References68
  • Citations15

Mentioned in this Paper

Embryo
Lactate
Entire Embryo
Amino Acids, I.V. solution additive
Protoplasm
Lactic Acid Measurement
Lactates
Fertilization in Vitro
Gametes
Bicarbonate Measurement

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