Is thermosensing property of RNA thermometers unique?

PloS One
Premal Shah, Michael A Gilchrist

Abstract

A large number of studies have been dedicated to identify the structural and sequence based features of RNA thermometers, mRNAs that regulate their translation initiation rate with temperature. It has been shown that the melting of the ribosome-binding site (RBS) plays a prominent role in this thermosensing process. However, little is known as to how widespread this melting phenomenon is as earlier studies on the subject have worked with a small sample of known RNA thermometers. We have developed a novel method of studying the melting of RNAs with temperature by computationally sampling the distribution of the RNA structures at various temperatures using the RNA folding software Vienna. In this study, we compared the thermosensing property of 100 randomly selected mRNAs and three well known thermometers--rpoH, ibpA and agsA sequences from E. coli. We also compared the rpoH sequences from 81 mesophilic proteobacteria. Although both rpoH and ibpA show a higher rate of melting at their RBS compared with the mean of non-thermometers, contrary to our expectations these higher rates are not significant. Surprisingly, we also do not find any significant differences between rpoH thermometers from other gamma-proteobacteria and E. coli ...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M H de Smit, J van Duin
Apr 18, 1997·Journal of Molecular Biology·M HuynenD Konings
Oct 17, 2001·Journal of Molecular Biology·R K ShultzabergerT D Schneider
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan A BernsteinStanley N Cohen
Jul 29, 2003·Molecular Genetics and Genomics : MGG·G HambraeusL Hederstedt
Sep 10, 2003·The Journal of Biological Chemistry·Saheli ChowdhuryFranz Narberhaus
Feb 14, 2004·Bioinformatics·Björn VossRobert Giegerich
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·David H MathewsDouglas H Turner
Dec 13, 2005·Biological Chemistry·Torsten WaldminghausFranz Narberhaus
Jan 28, 2006·FEMS Microbiology Reviews·Franz NarberhausSaheli Chowdhury
May 20, 2006·The EMBO Journal·Saheli ChowdhuryFranz Narberhaus
Jul 25, 2007·Molecular Genetics and Genomics : MGG·Torsten WaldminghausFranz Narberhaus
Apr 11, 2009·Science·Grzegorz KudlaJoshua B Plotkin
Mar 1, 2006·Computational and Mathematical Methods in Medicine·T A Hughes, J N McElwaine

❮ Previous
Next ❯

Citations

Jul 6, 2012·Nanoscale·Carlos D S BritesLuís D Carlos
Apr 12, 2011·Molecular Microbiology·Laurène BastetDaniel A Lafontaine
Mar 28, 2017·Journal of Thermal Biology·Basavaraj SajjanarNarendra Pratap Singh
Mar 17, 2012·Nature Reviews. Microbiology·Jens Kortmann, Franz Narberhaus
Oct 1, 2020·Angewandte Chemie·Obianuju A NwokoloIrina V Nesterova

❮ Previous
Next ❯

Software Mentioned

RNAsubopt
R
Vienna
RNA

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.