Is toxicant-induced Sertoli cell injury in vitro a useful model to study molecular mechanisms in spermatogenesis?

Seminars in Cell & Developmental Biology
Nan LiC Yan Cheng

Abstract

Sertoli cells isolated from rodents or humans and cultured in vitro are known to establish a functional tight junction (TJ)-permeability barrier that mimics the blood-testis barrier (BTB) in vivo. This model has been widely used by investigators to study the biology of the TJ and the BTB. Studies have shown that environmental toxicants (e.g., perfluorooctanesulfonate (PFOS), bisphenol A (BPA) and cadmium) that exert their disruptive effects to induce Sertoli cell injury using this in vitro model are reproducible in studies in vivo. Thus, this in vitro system provides a convenient approach to probe the molecular mechanism(s) underlying toxicant-induced testis injury but also to provide new insights in understanding spermatogenesis, such as the biology of cell adhesion, BTB restructuring that supports preleptotene spermatocyte transport, and others. Herein, we provide a brief and critical review based on studies using this in vitro model of Sertoli cell cultures using primary cells isolated from rodent testes vs. humans to monitor environmental toxicant-mediated Sertoli cell injury. In short, recent findings have shown that environmental toxicants exert their effects on Sertoli cells to induce testis injury through their action o...Continue Reading

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Citations

Apr 25, 2020·Molecular Reproduction and Development·Massimo VendittiImed Messaoudi
May 11, 2018·DNA and Cell Biology·Juan Ramos-TreviñoMartha P Nava-Hernández
Jun 30, 2019·Current Molecular Pharmacology·Salvatore BenvengaLetteria Minutoli
Jun 6, 2021·Toxicology and Applied Pharmacology·A S VallésN E Furland

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