Is TRAP-6 suitable as a positive control for platelet reactivity when assessing response to clopidogrel?

Platelets
T GremmelS Panzer

Abstract

Adenosine 5′-diphosphate (ADP) inducible aggregation is used to assess platelet response to thienopyridines. Thrombin receptor-activating peptide-6 (TRAP-6) inducible aggregation may serve as a positive control because it acts via the thrombin receptor protease-activating receptor-1, which is not blocked by thienopyridines. We therefore investigated if TRAP-6 is suitable as a positive control when assessing residual platelet reactivity to ADP. Platelet response to clopidogrel was assessed in 200 patients on dual antiplatelet therapy using ADP inducible platelet aggregation by light transmission aggregometry (LTA), multiple electrode aggregometry (MEA), and the shear-dependent Impact-R. Test specificities were monitored by TRAP-6 inducible platelet aggregation. The aggregation-independent vasodilator-stimulated phosphoprotein (VASP) phosphorylation assay served for comparisons. ADP inducible aggregation was correlated to that by TRAP-6 (r = 0.33 to 0.72; p < 0.001 for all assays). A linear correlation was seen within MEA (r = 0.72). LTA TRAP-6 correlated weakly with the VASP assay (r = 0.19; p = 0.01), while there were no correlations of TRAP-6 responses by MEA or the Impact-R with the VASP assay (r = 0.03 and &#x2212;0.09; p > ...Continue Reading

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Citations

Jan 15, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Roza Badr EslamSimon Panzer
Aug 30, 2014·Clinical Hemorheology and Microcirculation·Marco RanucciSanti Trimarchi
Sep 12, 2014·British Journal of Anaesthesia·M RanucciUNKNOWN Surgical and Clinical Outcome Research (SCORE) Group
Oct 9, 2012·International Journal of Cardiology·Roza Badr EslamThomas Gremmel
Jul 9, 2016·Journal of Thrombosis and Thrombolysis·Jonathan RilingerChristoph B Olivier
Jun 24, 2017·Biomedical Research·Hideaki SuzukiTakafumi Ichikawa
Jul 12, 2019·Research and Practice in Thrombosis and Haemostasis·Patricia P WadowskiThomas Gremmel
Oct 28, 2020·Journal of Cardiovascular Pharmacology and Therapeutics·Patricia P WadowskiThomas Gremmel
Nov 19, 2020·Vascular Pharmacology·Silvia LeeThomas Gremmel

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