IS1533-based PCR assay for identification of Leptospira interrogans sensu lato serovars.

Journal of Clinical Microbiology
Richard L ZuernerC A Bolin

Abstract

A PCR-based assay was developed for typing L. interrogans sensu lato serovars. The assay is designed to exploit the presence of many copies of the leptospiral insertion sequence IS1533 and IS1533-like sequences present in the genomes of most leptospiral serovars. The PCR primers were designed to amplify DNA of unknown sequence between closely placed IS1533 or IS1533-like sequences. Amplification reactions primed with IS1533-based primers generated products of different sizes. When few copies of IS1533 were present in the genome, amplification of a few products was still detected. These results suggest that IS1533 elements may be found close together. Analysis of DNA amplified from different serovars showed the presence of differently sized products, thus enabling the serovars to be identified. Genetic variation among isolates within the same serovar was also demonstrated with the IS1533-based primers. Amplification reactions using DNA extracted from the urine of infected animals generated specific products which were similar to the products generated from purified bacterial DNA. These results demonstrate that this assay is selective enough to be used for typing leptospiral serovars from clinical material and thus allows leptosp...Continue Reading

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Citations

Jul 21, 2005·New Zealand Veterinary Journal·J S O'Keefe
Nov 17, 2011·PLoS Neglected Tropical Diseases·Ahmed AhmedSharon J Peacock
Dec 1, 2004·Journal of Travel Medicine·Jiri F P WagenaarRudy A Hartskeerl
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Morgan L HotkaDavid A Miller
Apr 9, 2001·Clinical Microbiology Reviews·P N Levett
Apr 20, 2002·Current Opinion in Infectious Diseases·J M Vinetz
Oct 6, 2006·FEMS Microbiology Letters·Raven E Reitstetter
Oct 19, 2020·The American Journal of Tropical Medicine and Hygiene·Christine AtherstoneSiobhan M Mor
Apr 16, 2016·Indian Journal of Medical Microbiology·R NarayananK Natarajaseenivasan
Feb 19, 1998·Analytical Biochemistry·T H WooM F Dohnt
Oct 8, 1997·Journal of Clinical Microbiology·R L Zuerner, C A Bolin

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