Abstract
To investigate the regulation of cell-to-cell coupling in myocardial ischaemia, the three-dimensional expression of connexin43 (Cx43) during experimental ischaemia was examined using a confocal laser scanning microscope. After induction of myocardial infarction in rats, serial optical sections were obtained from the left ventricular myocardium at various times (3 h to 60 days after ligation). The expression of Cx43 was detected immunohistochemically with FITC-labelled anti-rat Cx43 antibody. Fluorescent dots of Cx43 remained along the intercalated disc and decreased in number around the infarct up to 12 h after ligation. Cx43-expression disappeared completely within 48 h after ligation. After day 4, and especially on days 8 and 15 after ligation, the edges of the cardiomyocytes bordering the infarcted area manifested numerous sarcoplasmic tentacles that reacted positively to anti-desmin antibody. Distinct expression of Cx43 was observed extensively on the tentacles, although no cardiomyocytes remained viable around them. By day 60 after ligation, atypical expression of Cx43 had disappeared. These findings suggest that ischaemia induces temporally abnormal expression of Cx43, which might be responsible for abnormal conduction ar...Continue Reading
Citations
May 24, 2005·Cardiovascular Research·Kenneth W HewettRobert G Gourdie
Sep 20, 2008·Pathology International·Tetsuro Takamatsu
Jan 19, 2005·Japanese Heart Journal·Kazuhito HatanakaKen-ichi Yoshida
Aug 31, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Haley W SinnJim J-C Lin
Aug 16, 2011·Biochimica Et Biophysica Acta·Magda S C FontesHarold V M van Rijen
Apr 29, 2000·Circulation Research·M UzzamanI Kodama
Nov 26, 1999·Circulation Research·T MatsushitaT Takamatsu
Oct 5, 2002·Circulation Research·Ralph J Barker, Robert G Gourdie
Feb 2, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Sharon A GeorgeSteven Poelzing
Apr 13, 2007·Physiological Reviews·Stanley NattelYung-Hsin Yeh
May 8, 2019·Journal of Cellular and Molecular Medicine·Zhong-Hao LinJia-Feng Lin
Jan 25, 2018·International Journal of Legal Medicine·Aleksandra AljaknaSara Sabatasso
May 13, 2017·International Journal of Legal Medicine·Sara SabatassoTony Fracasso
Jul 7, 2018·Yonsei Medical Journal·Hyewon ParkBoyoung Joung
Apr 25, 2014·Journal of Cellular and Molecular Medicine·Xin LiBaofeng Yang
Mar 21, 2018·International Journal of Legal Medicine·Aleksandra AljaknaAurélien Thomas