Ischemia-induced activation of AMPK does not increase glucose uptake in glycogen-replete isolated working rat hearts

American Journal of Physiology. Heart and Circulatory Physiology
Mohamed A OmarAlexander S Clanachan

Abstract

Alterations in myocardial glucose metabolism are a key determinant of ischemia-induced depression of left ventricular mechanical function. Since myocardial glycogen is an important source of endogenous glucose, we compared the effects of ischemia on glucose uptake and utilization in isolated working rat hearts in which glycogen content was either replete (G replete, 114 micromol/g dry wt) or partially depleted (G depleted, 71 mumol/g dry wt). The effects of low-flow ischemia (LFI, 0.5 ml/min) on glucose uptake, glycogen turnover (glycogenolysis and glycogen synthesis), glycolysis, adenosine 5'-monophosphate-activated protein kinase (AMPK) activity, and GLUT4 translocation were measured. Relative to preischemic values, LFI caused a time-dependent reduction in glycogen content in both G-replete and G-depleted groups due to an acceleration of glycogenolysis (by 12-fold and 6-fold, respectively). In G-replete hearts, LFI (15 min) decreased glucose uptake (by 59%) and did not affect GLUT4 translocation. In G-depleted hearts, LFI also decreased initially glucose uptake (by 90%) and glycogen synthesis, but after 15 min, when glycogenolysis slowed due to exhaustion of glycogen content, glucose uptake increased (by 31%) in association w...Continue Reading

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Citations

Jan 8, 2010·Cardiovascular Research·Mohamed A OmarAlexander S Clanachan
Jul 4, 2012·Molecular and Cellular Endocrinology·Jeevan NagendranJason R B Dyck
Apr 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Manoj GandhiAlexander S Clanachan
Sep 2, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Prasanth PuthanveetilBrian Rodrigues
Jun 6, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Brandy PattersonRichard P Shannon

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