Ischemia-reperfusion injury in skeletal muscle: CD 18-dependent neutrophil-endothelial adhesion and arteriolar vasoconstriction

Plastic and Reconstructive Surgery
W A ZamboniR C Russell

Abstract

The purpose of this study was to evaluate if the venular neutrophil-endothelial adhesion associated with ischemia-reperfusion of skeletal muscle is dependent on leukocyte adhesion glycoprotein CD18 function and to determine if this interaction influences the vasoactive response in nearby arterioles. An in vivo microscopy preparation of transilluminated gracilis muscle in 13 male Wistar rats was used for this experiment. Observations of nonischemic muscle (sham) demonstrated this preparation to be stable for 8 hours with negligible change in neutrophil adherence or arteriole diameter. Three groups were evaluated in this study: (1) sham, no ischemia, no treatment (n = 5, 20 arterioles. 20 venules), (2) 4 hours of global ischemia only (n = 4, 19 venules, 22 arterioles), and (3) 4 hours of ischemia plus monoclonal antibody against CD18 (n = 4, 12 venules, 9 arterioles). The murine monoclonal antibody (WT.3, Seikagaku America, Inc.), which binds the rat leukocyte function antigen I CD18 chain, was infused into the contralateral femoral vein 30 minutes prior to reperfusion. The number of leukocytes rolling and adherent to endothelium (15 seconds of observation) was counted in 100-microns venular segments, and arteriole diameters were...Continue Reading

References

Sep 1, 1990·Journal of the American Society of Nephrology : JASN·R S Cotran, J S Pober
Dec 1, 1993·Toxicology and Applied Pharmacology·S R Thom

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Citations

Sep 26, 2000·Head & Neck·W R Carroll, R M Esclamado
Nov 20, 1998·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·M M al-Qattan
Jul 31, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·N R. Harris, R E. Rumbaut
Oct 8, 1999·Plastic and Reconstructive Surgery·P J MazolewskiW A Zamboni
Apr 1, 2000·Plastic and Reconstructive Surgery·J L LarsonW A Zamboni
Apr 17, 2001·Plastic and Reconstructive Surgery·T P OlivasW A Zamboni
Aug 24, 2005·Inflammatory Bowel Diseases·Norman R HarrisRobert D Specian
Apr 29, 2004·Plastic and Reconstructive Surgery·Allen GabrielWilliam A Zamboni
Aug 4, 2010·Plastic and Reconstructive Surgery·Seth R JonesWilliam A Zamboni
Mar 5, 2008·The Journal of Surgical Research·Kayvan T KhiabaniWilliam A Zamboni
Aug 25, 2000·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·A CicconettiA Romaniello
Apr 18, 2018·Clinical Hemorheology and Microcirculation·Laura PetrovicsGabor Jancso
Sep 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Min-Ho KimNorman R Harris
Apr 4, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Min-ho Kim, Norman R Harris
Mar 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Claudio ContaldoGuido A Wanner
Mar 20, 2003·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Norman R Harris
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert L Hester, Leah W Hammer
Dec 17, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Kavitha Nellore, Norman R Harris
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·K MiyakoshiY Yoshimura
Mar 9, 2019·Free Radical Biology & Medicine·Hong YuRonald J Korthuis
Apr 22, 2006·Annales de chirurgie plastique et esthétique·A OufquirJ Baudet

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