PMID: 8455573Feb 17, 1993Paper

Ischemic changes in myocardial ionic contents of the isolated perfused rat hearts as studied by NMR

Molecular and Cellular Biochemistry
T IshibashiS Imai

Abstract

Using 31P-, 23Na- and 39K-NMR, we assessed ischemic changes in high energy phosphates and ion contents of isolated perfused rat hearts continuously and systematically. To discriminate intra- and extracellular Na+, a shift reagent (Dy(TTHA)3-) was used in 23Na-NMR study. In 39K-NMR study, the extracellular K+ signal was suppressed by inversion recovery pulse sequence in order to obtain intracellular K+ signal without using shift reagents. During the early period of ischemia, increases in intracellular Na+ and inorganic phosphate (Pi) were observed in addition to the well-documented decreases in creatine phosphate and ATP and a fall of intracellular pH, suggesting an augmented operation of Na(+)-H+ exchange triggered by a fall of the intracellular pH resulted from breakdown of ATP. At around 15 min of ischemia, a second larger increase in intracellular Na+ and a decrease in intracellular K+ were observed in association with a second increase in Pi. This was accompanied by an abrupt rise of the ventricular end-diastolic pressure. As there was a depletion of ATP at this time, the increase in intracellular Na+ and associated decrease in intracellular K+ may be explained by inhibition of the Na(+)-K+ ATPase due to the depletion of AT...Continue Reading

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Citations

Nov 1, 1996·Pacing and Clinical Electrophysiology : PACE·M FukutaT Kobayashi
Aug 18, 2004·Journal of Neurosurgery·Prajak SaesueKazuhiro Hongo
Oct 1, 1995·Bioscience Reports·H Wolosker, L de Meis
Dec 1, 1996·Journal of Applied Physiology·V D SchepkinT F Budinger
Sep 1, 1993·Basic Research in Cardiology·W Scholz, U Albus

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