Ischemic nucleotide breakdown increases during cardiac development due to drop in adenosine anabolism/catabolism ratio

Journal of Molecular and Cellular Cardiology
J W de JongB Schoutsen

Abstract

Our earlier work on reperfusion showed that adult rat hearts released almost twice as much purine nucleosides and oxypurines as newborn hearts did [Am J Physiol 254 (1988) H1091]. A change in the ratio anabolism/catabolism of adenosine could be responsible for this effect. We therefore measured the activity of adenosine kinase, adenosine deaminase, nucleoside phosphorylase and xanthine oxidoreductase in homogenates of hearts and myocytes from neonatal and adult rats. In hearts the activity of adenosine deaminase and nucleoside phosphorylase (10-20 U/g protein) changed relatively little. However, adenosine kinase activity decreased from 1.3 to 0.6 U/g (P less than 0.025), and xanthine oxidoreductase activity increased from 0.02 to 0.85 U/g (P less than 0.005). Thus the ratio in activity of these rate-limiting enzymes for anabolism and catabolism dropped from 68 to 0.68 during cardiac development. In contrast, the ratio in myocytes remained unchanged (about 23). The large difference in adenosine anabolism/catabolism ratio, observed in heart homogenates, could explain why ATP breakdown due to hypoxia is lower in neonatal than in adult heart. Because this change is absent in myocytes, we speculate that mainly endothelial activities...Continue Reading

Citations

Sep 1, 1991·Pflügers Archiv : European journal of physiology·T P Geisbuhler, M J Rovetto
Feb 23, 1995·Molecular and Cellular Biochemistry·R T SmolenskiA M Seymour
Apr 11, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Ewa M SlominskaJulian Swierczynski
Oct 29, 2003·Experimental Gerontology·Laura WillemsJohn P Headrick
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Jun 26, 1998·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·C C GrayM H Yacoub

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