Ischemic preconditioning and morphine attenuate myocardial apoptosis and infarction after ischemia-reperfusion in rabbits: role of delta-opioid receptor

American Journal of Physiology. Heart and Circulatory Physiology
Shinji OkuboNoboru Takekoshi

Abstract

We examined whether ischemic preconditioning (IPC) attenuates ischemia-reperfusion injury, in part, by decreasing apoptosis and whether the delta-opioid receptor (DOR) plays a pivotal role in the regulation of apoptosis. Rabbits were subjected to 30-min coronary artery occlusion (CAO) and 180 min of reperfusion. IPC was elicited with four cycles of 5-min ischemia and 10-min reperfusion before CAO. Morphine (0.3 mg/kg iv) was given 15 min before CAO. Naloxone (Nal; 10 mg/kg iv) and naltrindole (Nti; 10 mg/kg iv), the respective nonselective and selective DOR antagonists were given 10 min before either morphine or IPC. Infarct size (%risk area) was reduced from 46 +/- 3.8 in control to 11.6 +/- 1.0 in IPC and 19.5 +/- 3.8 in the morphine group (means +/- SE; P < 0.001 vs. control). Nal blocked the protective effects of IPC and morphine, as shown by the increase in infarct size to 38.6 +/- 7.2 and 44.5 +/- 1.8, respectively. Similarly, Nti blocked IPC and morphine-induced protection. The percentage of apoptotic cells (revealed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay) decreased in IPC (3.6 +/- 1.9) and morphine groups (5.2 +/- 1.2) compared with control group (12.4 +/- 1.6; P < 0.001). Nti pre...Continue Reading

References

Oct 1, 1995·Hypertension·P HametJ Tremblay
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BuerkeA M Lefer
May 1, 1995·The American Journal of Physiology·J E SchultzG J Gross
Oct 1, 1994·The Journal of Clinical Investigation·R A GottliebR L Engler
Dec 1, 1995·European Heart Journal·P AnversaG Olivetti
Nov 1, 1996·Circulation Research·H Fliss, D Gattinger
Mar 1, 1997·Journal of Molecular and Cellular Cardiology·J E SchultzR C Kukreja
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S OkuboR C Kukreja
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J HuhB T Liang
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·R M FryerG J Gross
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·R SchulzG Heusch
Jul 11, 2001·Apoptosis : an International Journal on Programmed Cell Death·Z Q ZhaoJ Vinten-Johansen
Jun 11, 2002·Journal of Molecular and Cellular Cardiology·Mihaela M MocanuDerek M Yellon
Jun 24, 2003·Journal of Molecular and Cellular Cardiology·Garrett J Gross
Jul 19, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Koh KuzumeDonna M Van Winkle

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Citations

Mar 6, 2012·Journal of Molecular Neuroscience : MN·Farzaneh RostamiLeila Dargahi
Oct 15, 2013·European Journal of Pharmacology·Stylianos DragasisTheodoros Xanthos
Jun 21, 2008·Clinical and Experimental Pharmacology & Physiology·Rouhollah Habibey, Hamidreza Pazoki-Toroudi
Jul 6, 2010·Free Radical Biology & Medicine·Rouhollah HabibeyHamidreza Pazoki-Toroudi
Dec 25, 2007·The Journal of Surgical Research·Zuolei ChenBingxi Zhang
Jul 26, 2005·Peptides·Richard J Bodnar, Gad E Klein
Dec 8, 2011·Korean journal of anesthesiology·Jin Mo KimJun Kim
Jun 10, 2011·Journal of Cardiovascular Pharmacology·Hai-Tao GuoJian-Ming Pei
Aug 19, 2009·Fundamental & Clinical Pharmacology·Hamid Reza Pazoki-ToroudiRouhollah Habibey
Feb 4, 2006·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Christian MühlfeldHilmar Dörge
Dec 2, 2005·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Fernando Lopez-NeblinaLuis H Toledo-Pereyra
Jan 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Amanda J ZattaJakob Vinten-Johansen
Aug 31, 2019·Journal of Basic and Clinical Physiology and Pharmacology·Naseer Ahmed
Oct 31, 2015·Anesthesia and Analgesia·Daisuke MaruyamaMichiaki Yamakage
Feb 3, 2015·Experimental Physiology·Lindsey E MillerJohn C Quindry
May 21, 2016·Medicinal Research Reviews·Leonid N MaslovJames M Downey
Jul 1, 2017·European Journal of Clinical Pharmacology·Nicholas T VozorisPaula A Rochon
Apr 4, 2021·International Journal of Molecular Sciences·Christopher LotzFranz Kehl
Apr 26, 2011·Journal of Cardiothoracic and Vascular Anesthesia·Kook Jin ChunMi Young Lee
Oct 31, 2020·European Journal of Pharmacology·Li-Ning WuJun-Ma Yu

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