Ischemic preconditioning improves mitochondrial tolerance to experimental calcium overload

The Journal of Surgical Research
J A CrestanelloM D Osbakken

Abstract

Ca(2+) overload leads to mitochondrial uncoupling, decreased ATP synthesis, and myocardial dysfunction. Pharmacologically opening of mitochondrial K(ATP) channels decreases mitochondrial Ca(2+) uptake, improving mitochondrial function during Ca(2+) overload. Ischemic preconditioning (IPC), by activating mitochondrial K(ATP) channels, may attenuate mitochondrial Ca(2+) overload and improve mitochondrial function during reperfusion. The purpose of these experiments was to study the effect of IPC (1) on mitochondrial function and (2) on mitochondrial tolerance to experimental Ca(2+) overload. Rat hearts (n = 6/group) were subjected to (a) 30 min of equilibration, 25 min of ischemia, and 30 min of reperfusion (Control) or (b) two 5-min episodes of ischemic preconditioning, 25 min of ischemia, and 30 min of reperfusion (IPC). Developed pressure (DP) was measured. Heart mitochondria were isolated at end-Equilibration (end-EQ) and at end-Reperfusion (end-RP). Mitochondrial respiratory function (state 2, oxygen consumption with substrate only; state 3, oxygen consumption stimulated by ADP; state 4, oxygen consumption after cessation of ADP phosphorylation; respiratory control index (RCI, state 3/state 4); rate of oxidative phosphorylat...Continue Reading

References

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Sep 1, 1996·The Journal of Surgical Research·J A CrestanelloG J Whitman
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Apr 13, 1999·Journal of Molecular and Cellular Cardiology·N M DolibaM D Osbakken
Dec 6, 2000·The Journal of Surgical Research·J A CrestanelloG J Whitman

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Citations

Mar 19, 2008·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·David Wright, Lindsey Sutherland
Jul 6, 2012·The Journal of Surgical Research·Ricardo QuarrieJuan A Crestanello
Aug 8, 2007·Pharmacology & Therapeutics·Derek J Hausenloy, Derek M Yellon
Mar 21, 2006·Archives of Biochemistry and Biophysics·Kathleen C Lundberg, Luke I Szweda
Mar 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Miomir JovicBato Korac
Aug 3, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Ricardo QuarrieJuan A Crestanello
Apr 7, 2007·Journal of Cardiovascular Medicine·Naranjan S DhallaSuresh K Gupta
Mar 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Mirian M da SilvaAlicia J Kowaltowski
Dec 14, 2019·Indian Journal of Pharmacology·Lamiaa Ahmed Ahmed
Dec 9, 2003·European Journal of Pharmacology·Pedro MonteiroLuís A Providência

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