Ischemic Preconditioning Mediates Neuroprotection against Ischemia in Mouse Hippocampal CA1 Neurons by Inducing Autophagy

PloS One
Chunlin GaoJialing Wu

Abstract

The hippocampal CA1 region is sensitive to hypoxic and ischemic injury but can be protected by ischemic preconditioning (IPC). However, the mechanism through which IPC protects hippocampal CA1 neurons is still under investigation. Additionally, the role of autophagy in determining the fate of hippocampal neurons is unclear. Here, we examined whether IPC induced autophagy to alleviate hippocampal CA1 neuronal death in vitro and in vivo with oxygen glucose deprivation (OGD) and bilateral carotid artery occlusion (BCCAO) models. Survival of hippocampal neurons increased from 51.5% ± 6.3% in the non-IPC group (55 min of OGD) to 77.3% ± 7.9% in the IPC group (15 min of OGD, followed by 55 min of OGD 24 h later). The number of hippocampal CA1 layer neurons increased from 182 ± 26 cells/mm2 in the non-IPC group (20 min of BCCAO) to 278 ± 55 cells/mm2 in the IPC group (1 min × 3 BCCAO, followed by 20 min of BCCAO 24 h later). Akt phosphorylation and microtubule-associated protein light chain 3 (LC3)-II/LC3-I expression were increased in the preconditioning group. Moreover, the protective effects of IPC were abolished only by inhibiting the activity of autophagy, but not by blocking the activation of Akt in vitro. Using in vivo experime...Continue Reading

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Citations

Jul 6, 2019·Frontiers in Neuroscience·Audrey M ThiebautBenoit D Roussel
Mar 3, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Jin-Lan HuangZhen-Guo Zhong
Jan 29, 2019·Frontiers in Physiology·Wen-Ting LiaoWen-Yuan Liu
Aug 10, 2017·Journal of Neuropathology and Experimental Neurology·Fengyan ZhaoDezhi Mu
Nov 3, 2020·Frontiers in Cellular Neuroscience·Jie LiuYi Yang
Mar 11, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Meng WangYuchuan Ding
Oct 3, 2018·The Annals of Thoracic Surgery·Lihui YanHongbo Liu

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

SPSS

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