Ischemic preconditioning protects the brain against injury via inhibiting CaMKII-nNOS signaling pathway

Brain Research
Mei WangGuang-Yi Zhang

Abstract

Although studies have shown that cerebral ischemic preconditioning (IPC) can ameliorate ischemia/reperfusion (I/R) induced brain damage, but its precise mechanisms remain unknown. Therefore, the aim of this study was to investigate the neuroprotective mechanisms of IPC against ischemic brain damage induced by cerebral I/R and to explore whether the Calcium/calmodulin-dependent protein kinase II (CaMKII)-mediated up-regulation of nNOS ser847-phosphorylation signaling pathway contributed to the protection provided by IPC. Transient global brain ischemia was induced by 4-vessel occlusion in adult male Sprague-Dawley rats. The rats were pretreated with 3 min of IPC alone or KN62 (selective antagonist of CaMKII) treatment before IPC, after reperfusion for 3 days, 6 min ischemia was induced. Cresyl violet staining was used to examine the survival of hippocampal CA1 pyramidal neurons. Immunoblotting was performed to measure the phosphorylation of CaMKII, nNOS, c-Jun and the expression of FasL. Immunoprecipitation was used to examine the binding between PSD95 and nNOS. The results showed that IPC could significantly protect neurons against cerebral I/R injury, furthermore, the combination of PSD95 and nNOS was increased, coinstantaneou...Continue Reading

References

Sep 24, 1990·Brain Research·K KitagawaK Mikoshiba
Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Feb 1, 1994·Stroke; a Journal of Cerebral Circulation·S K HansonP Ostrow
Jul 10, 1999·The Journal of Biological Chemistry·Y HayashiY Watanabe
Oct 31, 2000·Journal of the Neurological Sciences·B C WhiteG S Krause
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·C IkonomidouL Turski
Jul 1, 2006·Stroke; a Journal of Cerebral Circulation·Graeme J Hankey
Sep 15, 2006·The Journal of Pharmacology and Experimental Therapeutics·Dawn S CarterRobert J DeLorenzo
Mar 30, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gerald A RameauEdward B Ziff
Dec 29, 2009·Cell Calcium·Carlos Matute
Mar 6, 2012·Journal of the Neurological Sciences·Dashi QiYuanjian Song
Feb 14, 2013·Behavioural Brain Research·Régis Gemerasca MestrinerCarlos Alexandre Netto
Apr 29, 2014·Behavioural Brain Research·Xiangru WenYuanjian Song
May 13, 2014·International Immunopharmacology·Se-Eun JangDong-Hyun Kim
Oct 16, 2014·Behavioural Brain Research·Régis Gemerasca MestrinerCarlos Alexandre Netto
Jan 2, 2015·Dose-response : a Publication of International Hormesis Society·Roger Simon
Feb 24, 2015·Frontiers in Medicine·Arun Chaudhury
May 9, 2015·Journal of Neuroinflammation·Raffaela CiprianiCarlos Matute

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Citations

Dec 15, 2019·Archives of Physiology and Biochemistry·Rachid AkkiAngela Marino
Aug 10, 2017·Frontiers in Neuroscience·Oleg G DeryaginVladimir B Koshelev
Oct 31, 2020·International Journal of Molecular Sciences·Shoma ArakiYasuo Watanabe

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