ISCOM vaccine against contagious bovine pleuropneumonia (CBPP). 1. Biochemical and immunological characterization

Veterinary Immunology and Immunopathology
I AbusugraB Morein

Abstract

A better vaccine than the existing ones against contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) would improve the chances for eradication of CBPP. In such an effort, immunostimulating complexes (ISCOMS) have been prepared from the whole detergent-solubilized cells of MmmSC and characterized biochemically and immunologically. The most efficient detergent for solubilization of the mycoplasma was MEGA-10 which yielded a high recovery of proteins in the ISCOMS. The ISCOMS showed the typical cage-like structure by EM and sedimented as 19S by sucrose gradient centrifugation. The protein pattern of the ISCOMS, analyzed in SDS-PAGE, revealed a great number of bands distributed along the gel as high and low molecular weight polypeptides. The Western blot developed with a serum from a CBPP infected animal detected a reduced number of polypeptides. In samples from whole mycoplasma cells and in ISCOMS, lectin blots revealed more than 20 carbohydrate structures. The ISCOMS induced a strong primary antibody response in mice measured by ELISA and the boost resulted in a 6-fold increase of the serum antibody response. The IgG response was distributed into various IgG subclasses w...Continue Reading

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Citations

Nov 25, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·O J B HübschleB Morein
Jun 12, 2004·Advanced Drug Delivery Reviews·Bror MoreinIzzeldin Abusugra
Dec 30, 2008·Journal of Comparative Pathology·R A J NicholasL McAuliffe
May 20, 2009·Vaccine·Hong-Xiang SunYi-Ping Ye

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