PMID: 9649949Jul 3, 1998Paper

Islet cell surface antibodies (ICSA) do bind to human pancreas: computerised, quantitative determination of ICSA using both pre- and postembedding immunocolloidal techniques

Diabetes Research and Clinical Practice
B KniolaJ Ludvigsson

Abstract

Islet cell surface antibodies (ICSA) have been demonstrated significantly more often in serum of patients with IDDM and their relatives than in healthy controls, but some investigators have been unable to show binding to human islets. It has therefore been suggested that ICSA is an artefact. We have localised and quantified ICSA binding to different specimens, including human pancreas. ICSA-positive and ICSA-negative patients' serum, determined by a RIA-method, were incubated with ultrathin sections of rat insulinoma (RIN5AH) cells, stained with Goat-anti-human IgG conjugated with 10 nm gold particles. Sections were viewed in transmission electron microscopy (Mag. 30,000 x) and image analyses was used to calculate immunolabelling. To test cell specificity we used (RIN5AH-cells, rat tumour cells producing growth hormone (GH3), normal human pancreas, human insulinoma, and mice liver). Sections showed good morphology. ICSA-positive sera, with or without islet cell antibodies (ICA) gave always higher immunocolloidal labelling then ICSA-negative sera on RIN5AH sections. Thus, the colloidal gold technique could confirm the ICSA results determined with RIA. Immunolabelling was pronounced on normal human pancreas and human insulinoma c...Continue Reading

References

Aug 24, 1978·The New England Journal of Medicine·A LernmarkH S Traisman
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Citations

Dec 11, 2008·Journal of Gastroenterology and Hepatology·Antonio M Morselli-Labate, Raffaele Pezzilli
Dec 21, 2002·The British Journal of Nutrition·Irene L BlackMichael J Gibney
Nov 12, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rita RosenthalOlaf Strauss

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