Islet Endothelial Cells Derived From Mouse Embryonic Stem Cells

Cell Transplantation
Neha Jain, Eun Jung Lee

Abstract

The islet endothelium comprises a specialized population of islet endothelial cells (IECs) expressing unique markers such as nephrin and α-1 antitrypsin (AAT) that are not found in endothelial cells in surrounding tissues. However, due to difficulties in isolating and maintaining a pure population of these cells, the information on these islet-specific cells is currently very limited. Interestingly, we have identified a large subpopulation of endothelial cells exhibiting IEC phenotype, while deriving insulin-producing cells from mouse embryonic stem cells (mESCs). These cells were identified by the uptake of low-density lipoprotein (LDL) and were successfully isolated and subsequently expanded in endothelial cell culture medium. Further analysis demonstrated that the mouse embryonic stem cell-derived endothelial cells (mESC-ECs) not only express classical endothelial markers, such as platelet endothelial cell adhesion molecule (PECAM1), thrombomodulin, intercellular adhesion molecule-1 (ICAM-1), and endothelial nitric oxide synthase (eNOS) but also IEC-specific markers such as nephrin and AAT. Moreover, mESC-ECs secrete basement membrane proteins such as collagen type IV, laminin, and fibronectin in culture and form tubular net...Continue Reading

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Citations

Feb 17, 2018·Biofabrication·Monika HospodiukIbrahim T Ozbolat
Sep 15, 2018·Cell Transplantation·Dong-Won SeolDong Ryul Lee

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Methods Mentioned

BETA
IECs
IEC
Fluorescence-activated flow cytometry
FACS
enzyme-linked immunosorbent assay
ELISA
Assay

Software Mentioned

SPSS
FlowJo

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