Islet engraftment and revascularization in clinical and experimental transplantation

Cell Transplantation
Christian MolnárOlle Korsgren

Abstract

Proper revascularization after transplantation is assumed to be crucial for appropriate islet graft function. We developed a novel noninvasive imaging method, based on adenoviral transduction of islets with a hypoxia responsive reporter gene, for continuous in vivo monitoring of hypoxia in islet grafts in a mouse model. In addition, morphological data were obtained from a deceased patient previously subject to intraportal transplantation. We detected only transient hypoxia in a minority of the animals transplanted. Importantly, a clear response to hypoxia was observed in vitro after removal of the islet grafts on day 28 after transplantation. Also, the morphological data from the deceased patient demonstrated an extensive revascularization of the transplanted islets. In fact, no differences could be seen between native islets, in pancreas biopsies taken prior to islet isolation, and transplanted islets regarding the number, distribution, and shape of the blood vessels. However, fewer small islets (diameter <39 µm) were found in the liver compared to those found in native pancreases. Notably, an absolute majority of the transplanted islets were found remaining within the venous lumen, in direct contact with the vessel wall. In c...Continue Reading

References

Apr 1, 1988·Diabetes·C RicordiD W Scharp
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·M EdingerC H Contag
Nov 4, 2000·The Journal of Clinical Endocrinology and Metabolism·A M DavalliA Secchi
Nov 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D M StrokaD Candinas
Mar 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wolfgang MoritzMarkus Weber
Apr 30, 2002·Diabetes·Göran MattssonPer-Ola Carlsson
Jul 11, 2002·Diabetologia·L Jansson, P-O Carlsson
Oct 11, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda W WyattGiovanni E Mann
Dec 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Per-Ola CarlssonGöran Mattsson
Dec 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Boaz HirshbergDavid M Harlan
Sep 27, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·José OberholzerPhilippe Morel
Apr 13, 2004·Transplantation·Martin WijkstromBernhard J Hering
Aug 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Wing-Shing ChengMagnus Essand
Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew B AdamsChristian P Larsen
Apr 2, 2005·Journal of Radiation Research·Junye LiuMasahiro Hiraoka
Jun 29, 2005·Diabetes·Edmond A RyanA M James Shapiro
Nov 24, 2005·American Journal of Physiology. Endocrinology and Metabolism·R R MacGregorL Stehno-Bittel
Mar 17, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M KoulmandaT B Strom
Oct 20, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·G MiaoE Hathout
Mar 1, 2007·Diabetes·Roger LehmannGiatgen A Spinas
Mar 3, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eri MukaiHiroyuki Mizuguchi
Oct 5, 2007·Cell Transplantation·Erik von SethLeif Jansson
Aug 16, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Chan Joo YeomDong Soo Lee

❮ Previous
Next ❯

Citations

Oct 10, 2013·Clinical & Developmental Immunology·Andrew R PepperA M James Shapiro
Dec 21, 2013·Diabetes·Heshan PeirisClaire F Jessup
Aug 31, 2016·Frontiers in Immunology·Gustaf Christoffersson, Matthias G von Herrath
Mar 5, 2016·Biotechnology and Applied Biochemistry·Na LiXiaojun Ma
Oct 12, 2014·Current Opinion in Organ Transplantation·Adam KrzystyniakPiotr Trzonkowski
Feb 14, 2019·Scientific Reports·Dina MyasnikovaJunji Fukuda
Sep 9, 2017·Transplantation·Christian SchuetzUNKNOWN IPITA YIC Young Investigator Committee
Aug 2, 2019·International Journal of Surgery·Benjamin SmoodDavid K C Cooper
Oct 28, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Ji LeiJames F Markmann

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
light microscopy

Software Mentioned

LivingImage®

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.