Islet Microvasculature Alterations With Loss of Beta-cells in Patients With Type 1 Diabetes

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Joseph S CanzanoMartha Campbell-Thompson

Abstract

Islet microvasculature provides key architectural and functional roles, yet the morphological features of islets from patients with type 1 diabetes are poorly defined. We examined islet and exocrine microvasculature networks by multiplex immunofluorescence imaging of pancreases from organ donors with and without type 1 diabetes ( n=17 and n=16, respectively) and determined vessel diameter, density, and area. We also analyzed these variables in insulin-positive and insulin-negative islets of 7 type 1 diabetes donors. Control islet vessel diameter was significantly larger (7.6 ± 1.1 μm) compared with vessels in diabetic islets (6.2 ± 0.8 μm; p<0.001). Control islet vessel density (number/islet) was significantly lower (5.3 ± 0.6) versus diabetic islets (9.3 ± 0.2; p<0.001). Exocrine vessel variables were not significantly different between groups. Islets with residual beta-cells were comparable to control islets for both vessel diameter and density and were significantly different from insulin-negative islets within diabetic donors ( p<0.05). Islet smooth muscle actin area had a significant positive correlation with age in both groups ( p<0.05), which could negatively impact islet transplantation efficiency from older donors. The...Continue Reading

References

Dec 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Per-Ola CarlssonGöran Mattsson
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Over CabreraAlejandro Caicedo
Mar 10, 2007·World Journal of Surgery·Nikiforos Ballian, F Charles Brunicardi
Jan 29, 2008·Current Diabetes Reviews·Maria M ZanoneGiovanni Camussi
Feb 27, 2010·Diabetes·Domenico BoscoThierry Berney
Jul 5, 2011·Cell Metabolism·Rayner Rodriguez-DiazAlejandro Caicedo
May 16, 2012·Diabetes/metabolism Research and Reviews·Martha Campbell-ThompsonMark Atkinson
Jun 6, 2012·Journal of Visualized Experiments : JoVE·Martha L Campbell-ThompsonMark A Atkinson
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jun 20, 2013·JAMA : the Journal of the American Medical Association·Anette G ZieglerGeorge S Eisenbarth
Jul 31, 2013·Lancet·Mark A AtkinsonAaron W Michels
Jan 22, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Susan Bonner-WeirGordon C Weir
May 10, 2015·Diabetologia·Austin G Davis-Richardson, Eric W Triplett
Jul 29, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marcela BrissovaRebecca L Hull
Jul 29, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel T MeierSteven E Kahn
Nov 20, 2015·Diabetes·Martha Campbell-ThompsonMark A Atkinson
Apr 29, 2016·Upsala Journal of Medical Sciences·Leif JanssonPer-Ola Carlsson
Nov 5, 2016·Developmental Biology·D Berfin AzizogluOndine Cleaver
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Sep 8, 2017·Diabetes, Obesity & Metabolism·Kristie I Aamodt, Alvin C Powers

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Citations

May 12, 2019·Reviews in Endocrine & Metabolic Disorders·Laure Alexandre-HeymannEtienne Larger
Aug 16, 2019·Diabetologia·Willem StaelsNico De Leu
Sep 8, 2020·Diabetologia·Mark A AtkinsonKlaus H Kaestner
Sep 30, 2018·Current Diabetes Reports·Shiue-Cheng TangMartha Campbell-Thompson
Feb 2, 2021·Frontiers in Endocrinology·Luciana Mateus Gonçalves, Joana Almaça
Jan 21, 2021·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Timothy P FosterDesmond A Schatz
May 14, 2021·Frontiers in Endocrinology·Martha Campbell-Thompson, Shiue-Cheng Tang
Jun 23, 2021·Trends in Endocrinology and Metabolism : TEM·Andreas Alvin Purnomo SoetedjoAdrian Kee Keong Teo
Aug 11, 2021·Cancer Cell·Ming Y LuFaisal Mahmood
Apr 13, 2021·Endocrine Reviews·John T WalkerAlvin C Powers

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Methods Mentioned

BETA
SMA
pancreatectomy

Software Mentioned

GraphPad
FIJI
custom Python scripts
GraphPad Prism
Excel

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