iso-DGR sequences do not mediate binding of fibronectin N-terminal modules to adherent fibronectin-null fibroblasts.

The Journal of Biological Chemistry
Jielin XuDeane F Mosher

Abstract

Fibronectin (FN) without an RGD sequence (FN-RGE), and thus lacking the principal binding site for alpha5beta1 integrin, is deposited into the extracellular matrix of mouse embryos. Spontaneous conversion of (263)NGR and/or (501)NGR to iso-DGR possibly explains this enigma, i.e. ligation of iso-DGR by alphavbeta3 integrin may allow cells to assemble FN. Partial modification of (263)NGR to DGR or iso-DGR was detected in purified plasma FN by mass spectrometry. To test functions of the conversion, one or both NGR sequences were mutated to QGR in recombinant N-terminal 70-kDa construct of FN (70K), full-length FN, or FN-RGE. The mutations did not affect the binding of soluble 70K to already adherent fibroblasts or the ability of soluble 70K to compete with non-mutant FN or FN-RGE for binding to FN assembly sites. Non-mutant FN and FN-N263Q/N501Q with both NGRs mutated to QGRs were assembled equally well by adherent fibroblasts. FN-RGE and FN-RGE-N263Q/N501Q were also assembled equally well. Although substrate-bound 70K mediated cell adhesion in the presence of 1 mm Mn(2+) by a mechanism that was inhibited by cyclic RGD peptide, the peptide did not inhibit 70K binding to cell surface. Mutations of the NGR sequences had no effect on...Continue Reading

References

Jun 1, 1991·The Journal of Cell Biology·J E Schwarzbauer
Apr 4, 1995·Biochemistry·T P UgarovaE F Plow
Feb 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·S GodynaW S Argraves
Jan 1, 1996·The Journal of Cell Biology·K WennerbergR Fässler
Feb 28, 1998·The Journal of Biological Chemistry·H BultmannD M Peters
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SaoncellaP F Goetinck
Oct 6, 1999·Current Opinion in Cell Biology·J E Schwarzbauer, J L Sechler
Apr 27, 2000·Nature Cell Biology·E ZamirB Geiger
Apr 27, 2001·The Journal of Biological Chemistry·B R Tomasini-JohanssonD F Mosher
Oct 13, 2001·Molecular Biology of the Cell·S F RettaG Tarone
Nov 21, 2001·Nature Reviews. Molecular Cell Biology·B GeigerK M Yamada
Jun 20, 2002·Methods in Cell Biology·Deane F MosherDouglas S Annis
Sep 24, 2002·Journal of Cell Science·Roumen Pankov, Kenneth M Yamada
May 9, 2003·Nature·Ulrich Schwarz-LinekJennifer R Potts
Jul 15, 2003·Journal of Cell Science·Iwona Wierzbicka-Patynowski, Jean E Schwarzbauer
Jun 17, 2004·The Journal of Biological Chemistry·Eunnyung BaeDeane F Mosher
Aug 3, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Yong Mao, Jean E Schwarzbauer
Mar 29, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Bianca R Tomasini-JohanssonDeane F Mosher
May 13, 2006·Journal of Thrombosis and Haemostasis : JTH·J Cho, D F Mosher
Jul 15, 2006·Journal of Thrombosis and Haemostasis : JTH·J Cho, D F Mosher
Oct 4, 2006·The Journal of Biological Chemistry·Flavio CurnisAngelo Corti
Jun 27, 2007·The Journal of Cell Biology·Seiichiro TakahashiReinhard Fässler
Jul 4, 2007·The Journal of Biological Chemistry·Nicola A G MeenanJennifer R Potts
Oct 19, 2007·Current Opinion in Cell Biology·Ioannis Vakonakis, Iain D Campbell
May 16, 2008·The Journal of Biological Chemistry·Andrea SpitaleriGiovanna Musco
Jun 25, 2008·Blood·Angelo CortiRenata Pasqualini
Jul 1, 2008·Current Opinion in Cell Biology·Michael LeissReinhard Fässler
Jul 26, 2008·Journal of Cell Science·Rachel KinseyCay M Kielty
Aug 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Richard J BinghamJennifer R Potts
Sep 2, 2008·Cancer Research·Flavio CurnisAngelo Corti
Nov 28, 2008·Molecular Biology of the Cell·Laetitia SabatierDieter P Reinhardt
Apr 16, 2009·The Journal of Biological Chemistry·Ioannis VakonakisIain D Campbell

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Citations

Oct 16, 2010·The Journal of Biological Chemistry·Lisa M MaurerDeane F Mosher
Sep 29, 2011·The Journal of Biological Chemistry·Tomoo Ohashi, Harold P Erickson
Feb 22, 2012·The Journal of Biological Chemistry·Lisa M MaurerDeane F Mosher
Sep 20, 2011·Fibrogenesis & Tissue Repair·Wing S To, Kim S Midwood
Jan 15, 2014·European Journal of Medicinal Chemistry·Samantha D DattoliLuca Gentilucci
May 18, 2016·Critical Reviews in Biochemistry and Molecular Biology·Lisa M MaurerDeane F Mosher
Jan 19, 2016·Medicinal Research Reviews·Rossella De MarcoLuca Gentilucci
Nov 4, 2020·The Journal of Cell Biology·María Benito-JardónMercedes Costell
Feb 2, 2011·Journal of Cell Science·Angelo Corti, Flavio Curnis

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