Isoantigenicity of rabbit sperm, testis, and their extracts as demonstrated by Western blot enzyme immunobinding procedure

Archives of Andrology
R K NazA C Menge

Abstract

The isoantigenicity of rabbit germ cells and their extracts was studied by using a Western blot enzyme immunobinding procedure after systemic and local intrauterine immunization. In the deoxycholate (DOC) sperm extract, the antisperm isoantiserum (ASA) recognized proteins of 100, 96, 92, 82, 53, 47, 43, and 29 kD, and the antitestis isoantiserum (ATA) recognized proteins of 100, 96, 92, 82, 43, and 29 kD. In the lithium diiodosalicylate (LIS) sperm extract, ASA reacted with four antigen bands of 92, 30, 14, and 6 kD, and ATA reacted with proteins of 94, 92, 91, 23, 17, 7, and 6 kD. In the reduced sodium dodecyl sulfate (SDS) sperm extract, ASA recognized proteins of 95, 76, 51, 41, 33, 30, 28, 26, and 22 kD; ATA showed a reaction only with proteins of 100, 98, and 95 kD, and anti-LIS/sperm isoantiserum (ALA) recognized three proteins of 98, 81, and 65 kD. The IgA of the immune uterine fluid (IUF) from the LIS/sperm-immunized rabbits recognized a 14-kD antigen in the DOC conceptus extract, a 51-kD antigen in the DOC testis extract, and 53-, 49-, 31-, and 28-kD proteins in the reduced SDS-sperm pellet extract. The IgA of the IUF from the NP-40/pellet-immunized rabbits showed two proteins of about 100 kD, plus ones of 56, 36, 31, ...Continue Reading

References

Jul 1, 1977·The Journal of Cell Biology·C F Millette, A R Bellvé
Jun 1, 1977·Developmental Biology·M Hintz, E Goldberg
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1979·Journal of Reproductive Immunology·D B Morton, P A McAnulty
May 1, 1978·Biology of Reproduction·M G Munoz, C B Metz
Feb 1, 1977·Developmental Biology·M G O'Rand, L J Romrell
Aug 11, 1975·Differentiation; Research in Biological Diversity·I Radu, G A Voisin
Jun 1, 1973·Biology of Reproduction·C A Kiddy, R M Rollins
Jul 6, 1971·Biochimica Et Biophysica Acta·A H Maddy, P G Kelly
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·Y Hatefi, W G Hanstein
Jul 1, 1970·Journal of Reproduction and Fertility·E B Bell, A McLaren
Feb 1, 1983·Biology of Reproduction·R K NazA C Menge
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R K NazA C Menge
Feb 1, 1981·Fertility and Sterility·E GoldbergV C Stevens
Dec 1, 1964·Journal of Reproduction and Fertility·R E MANCINIM NEMIROVSKY

❮ Previous
Next ❯

Citations

Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R K NazB B Rosenblum
Sep 1, 1988·American Journal of Reproductive Immunology and Microbiology : AJRIM·A C Menge, R K Naz
Sep 18, 1998·Molecular Reproduction and Development·R K Naz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.