Isobavachalcone induces the apoptosis of gastric cancer cells via inhibition of the Akt and Erk pathways

Experimental and Therapeutic Medicine
Xiaohong Jin, Y I Shi

Abstract

In the present study, the MGC803 gastric cancer cell line was used as an experimental model to evaluate the potential role of isobavachalcone (IBC) in cell apoptosis, migration and invasion. The inhibitory effects of IBC on cell proliferation were determined using a methylthiazolyltetrazolium assay. Cellular morphological changes were assessed using Wright-Giemsa staining, and cell apoptosis was evaluated by flow cytometric analysis. The results of the present study demonstrated that IBC inhibited the proliferation of MGC803 cells in a concentration- and time-dependent manner. Furthermore, the wound healing and Matrigel™ Transwell® invasion assays demonstrated that IBC decreased cell migration and invasion in a concentration-dependent manner. Western blotting was used to determine the protein expression levels of caspase-3, B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax), as well as the key protein kinases in the Akt and extracellular signal-regulated kinase (Erk) signaling pathways. During the IBC-induced apoptosis of MGC803 cells, transient activation of phosphorylated (p)-Akt and p-Erk inhibited the activation of Akt and Erk, upregulated Bax expression, downregulated Bcl-2 expression and activated caspase-3. T...Continue Reading

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Citations

Apr 26, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Lanying MaSaifuding Keyoumu
May 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mengjun ShiWenyi Kang
Feb 13, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Miaojuan WangXiuping Chen
Jun 3, 2021·Cancers·Radka MichalkovaJan Mojzis
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Palko-ŁabuzOlga Wesołowska

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Methods Mentioned

BETA
protein assay
electrophoresis
Light microscopy

Software Mentioned

ImageJ2X
SPSS

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