Isocitrate dehydrogenase: A NADPH-generating enzyme in the lumen of the endoplasmic reticulum

Archives of Biochemistry and Biophysics
Eva Margittai, G Bánhegyi

Abstract

The aim of the present study was the investigation of the occurrence of NADPH-generating pathways in the endoplasmic reticulum others then hexose-6-phosphate dehydrogenase. A significant isocitrate and a moderate malate-dependent NADP+ reduction were observed in endoplasmic reticulum-derived rat liver microsomes. The isocitrate-dependent activity was very likely attributable to the appearance of the cytosolic isocitrate dehydrogenase isozyme in the lumen. The isocitrate dehydrogenase activity of microsomes was present in the luminal fraction; it showed a strong preference towards NADP+ versus NAD+, and it was almost completely latent. Antibodies against the cytosolic isoform of isocitrate dehydrogenase immunorevealed a microsomal protein of identical molecular weight; the microsomal enzyme showed similar kinetic parameters and oxalomalate inhibition as the cytosolic one. Measurable luminal isocitrate dehydrogenase activity was also present in microsomes from rat epididymal fat. The results suggest that isocitrate dehydrogenase is an important NADPH-generating enzyme in the endoplasmic reticulum.

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Citations

Dec 17, 2009·Antioxidants & Redox Signaling·Miklós CsalaGábor Bánhegyi
Mar 1, 2012·Antioxidants & Redox Signaling·Éva MargittaiGábor Bánhegyi
Dec 7, 2011·Antioxidants & Redox Signaling·Gábor BánhegyiMiklós Csala
Apr 30, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nadia A AtaiCornelis J F Van Noorden
Oct 7, 2009·Biological Chemistry·Silvia SenesiAngelo Benedetti
May 27, 2010·Redox Report : Communications in Free Radical Research·Daniela RogoffPerrin C White
Feb 4, 2010·Acta Neuropathologica·Fonnet E BleekerCornelis J F Van Noorden
Jun 1, 2010·Biochemical and Biophysical Research Communications·Yuejun FuAihua Liang
Jul 23, 2008·Journal of Neurochemistry·Gábor BánhegyiMiklós Csala
Oct 7, 2010·Journal of Diabetes·Tanya HyattKenneth McCormick
Feb 14, 2015·Free Radical Biology & Medicine·Éva MargittaiGábor Bánhegyi
Jul 29, 2016·Cancer Biology & Therapy·Jonathan R BrodyJordan M Winter
Aug 23, 2017·International Journal of Molecular Sciences·Alessandra GamberucciMarina Colombi
Aug 1, 2013·Physiological Reviews·Karen ChapmanJonathan Seckl
Apr 13, 2018·International Journal of Molecular Sciences·Ching-Ann LiuHorng-Jyh Harn
Jun 19, 2015·The Journal of Experimental Biology·Lars Tomanek
Jun 9, 2016·Open Access Macedonian Journal of Medical Sciences·Mohammed Sami Saeed
Jun 3, 2021·International Journal of Molecular Sciences·Isaac James MuyindaByong-Chul Yoo
May 31, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Paola MarcolongoAngelo Benedetti

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