Isocitrate dehydrogenase isozymes from a psychrotrophic bacterium, Pseudomonas psychrophila

Archives of Microbiology
Shougo MatsuoYasuhiro Takada

Abstract

The genes encoding monomer- and dimer-type isocitrate dehydrogenase (IDH) isozymes from a psychrotrophic bacterium, Pseudomonas psychrophila, were cloned and sequenced. Open reading frames of the genes were 2,226 and 1,257 bp in length and corresponded to polypeptides composed of 741 and 418 amino acids, respectively. The deduced amino acid sequences showed high sequence identity with those of psychrophilic bacteria, Colwellia maris and Colwellia psychrerythraea, (about 70% identity) and the respective types of the putative IDH genes from other bacteria of genus Pseudomonas (more than 80% identity). The two genes were located in opposite direction from each other with a spacer of 463 bases in the order of dimeric and monomeric IDH genes on the chromosomal DNA, but analyses of northern blotting and 5'-terminal regions of the mRNAs revealed that they are transcribed independently. The expression of monomer- and dimer-type IDH genes in C. maris are known to be cold- and acetate-inducible, respectively, while only slight inductions by low temperature and/or acetate were observed in the expression of the P. psychrophila monomer- and dimer-type IDH genes. Both of these IDH isozymes overproduced in Escherichia coli showed mesophilic p...Continue Reading

References

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Citations

Mar 12, 2011·Die Naturwissenschaften·Francesco Canganella, Juergen Wiegel
Apr 28, 2017·Extremophiles : Life Under Extreme Conditions·Ryo HirotaYasuhiro Takada

Related Concepts

Bacterial Proteins
DNA, Bacterial
Genes, Bacterial
Isocitrate Dehydrogenase-I
Alloenzymes
Pseudomonas
Mutagenesis, Site-Directed
Determination, Sequence Homology
Sequence Determinations, DNA
Transcription Initiation Site

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