Isocratic high performance liquid chromatographic analysis of S-adenosylmethionine and S-adenosylhomocysteine in animal tissues: the effect of exposure to nitrous oxide

Biomedical Chromatography : BMC
T Bottiglieri


A simple isocratic high performance liquid chromatographic method for the simultaneous measurement of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in animal tissues is described. The direct injection of perchloric acid tissue extracts and rapid resolution of both compounds in a single run reduces any sampling and analytical errors in determining the SAM/SAH ratio, a measure of methylation reactions. The method has been used to determine changes in brain SAM/SAH ratios after exposure of rats to nitrous oxide.


Jan 1, 1975·Journal of Neurochemistry·R A Schatz, O Z Sellinger
Mar 15, 1990·The Biochemical Journal·E Vieira-MakingsI Chanarin
Dec 1, 1988·Journal of Neurochemistry·D G WeirJ M Scott
Feb 7, 1981·Lancet·A R BosleyM E Dauncey
Aug 15, 1984·Analytical Biochemistry·G A MiuraP K Chiang
Jan 1, 1984·Advances in Experimental Medicine and Biology·P K Chiang
Feb 1, 1984·Analytical Biochemistry·P Giulidori, G Stramentinoli


Jul 1, 1997·Biological Trace Element Research·Eric O Uthus, R A Poellot
May 14, 2003·Experimental Gerontology·Eric O Uthus, Holly M Brown-Borg
Jan 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·Siming LiJiandie D Lin
Aug 21, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elango KathirvelTimothy R Morgan
Jun 24, 2008·American Journal of Physiology. Heart and Circulatory Physiology·S DayalS R Lentz
Apr 29, 2010·The Journal of Clinical Investigation·Bermans J IskandarKirk J Hogan
Jul 21, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Anne SchmedesIvan Brandslund
Jul 6, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·T BottiglieriEstelle Sontag
Jan 28, 2004·Alcoholism, Clinical and Experimental Research·Taunia D LeeShelly C Lu
Dec 12, 2012·Epigenetics : Official Journal of the DNA Methylation Society·Melissa B GlierAngela M Devlin
Jun 9, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jakub KrijtV Kozich
May 14, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ji ZhangMartin C Carey
Nov 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shelly C LuStephen J Pandol
Sep 7, 2007·Acta Biochimica Et Biophysica Sinica·Yideng JiangShuren Wang
May 19, 2005·The Journal of Biological Chemistry·Angela M DevlinS R Lentz
Mar 4, 2021·Medical Hypotheses·Andrew McCaddon, Björn Regland

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.