Isocratic high performance liquid chromatographic analysis of S-adenosylmethionine and S-adenosylhomocysteine in animal tissues: the effect of exposure to nitrous oxide

Biomedical Chromatography : BMC
T Bottiglieri

Abstract

A simple isocratic high performance liquid chromatographic method for the simultaneous measurement of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in animal tissues is described. The direct injection of perchloric acid tissue extracts and rapid resolution of both compounds in a single run reduces any sampling and analytical errors in determining the SAM/SAH ratio, a measure of methylation reactions. The method has been used to determine changes in brain SAM/SAH ratios after exposure of rats to nitrous oxide.

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