PMID: 8939402Dec 1, 1996Paper

Isoelectric focusing (IEF) and immunofixation for determination of disialotransferrin

Clinical Biochemistry
M F DumonM Clerc

Abstract

A new simplified method for detection and quantitation of disialontransferrin in serum is described. The method is based on polyacrylamide gel isoelectric focusing, direct immunofixation with a specific antibody, and measurement by computerized scanning densitometry. Disialotransferrin levels were determined in 24 teetotallers and 34 alcoholics at 3 moments during detoxification. Three groups of drinkers were arranged: group 1 (without), group 2 (with light), and group 3 (with severe hepatitis). The metho showed very good reproducibility and accuracy with a coefficient of variation between 5 to 8%. Alcoholic patients could be clearly separated from teetotallers, with a specificity of 100% and a sensitivity of 94%. After 12 days of alcohol withdrawal, disialotransferrin values declined in alcoholics but remained slightly high. They were not influenced by the severity of liver disease. No significant difference was found between the 3 groups. An easy-to-perform, sensitive, and inexpensive method has been developed to quantify disialotransferrin that can be used by laboratories almost everywhere.

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Citations

Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·F Musshoff, T Daldrup
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Frank Musshoff
Jul 21, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Eliska Marklová, Ziad Albahri
Apr 14, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shlomit Mesilaty-GrossGalia Gat-Yablonski
Aug 25, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Eliska Marklová, Ziad Albahri

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