PMID: 36141Apr 4, 1979

Isoelectric points of spinach thylakoid membrane surfaces as determined by cross partition

Biochimica Et Biophysica Acta
H E AkerlundP A Albertsson

Abstract

The isoelectric points of unbroken chloroplast lamellae and various subchloroplast fractions, including a preparation of inside-out thylakoids, have been determined using aqueous two-phase systems containing dextran and charged polyethylene glycol. When the amounts of material in the top phase in a phase system with the positively charged trimethylamino polyethylene glycol are plotted against pH the curve intersects the corresponding curve obtained from phase systems with the negatively charged polyethylene glycol sulfonate. This cross-point can be correlated with the isoelectric point of the material. The cross-point for unbroken chloroplast lamellae was found to be around pH 4.7. Mechanical disintegration lowered the cross-point to around pH 4.4, probably because of exposure of new membrane surfaces. The disintegrated chloroplasts were fractionated by differential centrifugation to separate the grana and stroma lamellae. The stroma lamellae vesicles showed the same isoelectric point as the unbroken lamellae, while a cross-point at pH 4.3 was obtained for the grana-enriched fraction. For thylakoid membranes destacked under low salt conditions the cross-point was 0.3 pH unit lower than for membranes originating exclusively from...Continue Reading

References

Jan 20, 1976·Biochimica Et Biophysica Acta·G Blomquist
Dec 6, 1976·Biochimica Et Biophysica Acta·H E AkerlundP A Albertsson
Dec 26, 1970·Nature·P A AlbertssonH Walter
Jul 3, 1967·Biochimica Et Biophysica Acta·R A Dilley, A Rothstein

Citations

Jan 1, 1986·Photosynthesis Research·K Satoh
Jul 9, 2011·The British Journal of Nutrition·Caroline MonteliusCharlotte Erlanson-Albertsson
May 1, 1983·Quarterly Reviews of Biophysics·S W Thorne, J T Duniec
Jan 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Inger CarlbergAlexander V Vener
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L R Griffing, R S Quatrano
Dec 22, 2009·Preparative Biochemistry & Biotechnology·Sinan C EmekCharlotte Erlanson-Albertsson
Oct 21, 2010·Journal of the Science of Food and Agriculture·Marilyn RaynerPer-Åke Albertsson
Jun 28, 2015·Plant Foods for Human Nutrition·Charlotte Erlanson-Albertsson, Per-Åke Albertsson
May 17, 2011·Biochimica Et Biophysica Acta·Alexander V RubanChristopher D P Duffy
Dec 20, 2016·Soft Matter·A Tamayo TenorioA J van der Goot
Mar 5, 2019·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Sahar Foshati, Maryam Ekramzadeh
Mar 20, 2004·EMBO Reports·Kentaro IfukuFumihiko Sato
Dec 27, 2016·Frontiers in Plant Science·Radek Kaňa, Govindjee

Related Concepts

Cell Fractionation
Plasma Membrane
Etioplasts
Hydrogen-Ion Concentration
Cell Surface Proteins
Plant Proteins
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