Isoepoxydon dehydrogenase (idh) gene expression in relation to patulin production by Penicillium expansum under different temperature and atmosphere

International Journal of Food Microbiology
N De ClercqE Van Coillie

Abstract

Penicillium expansum growth and patulin production occur mainly at post-harvest stage during the long-term storage of apples. Low temperature in combination with reduced oxygen concentrations is commonly applied as a control strategy to extend apple shelf life and supply the market throughout the year. Our in vitro study investigated the effect of temperature and atmosphere on expression of the idh gene in relation to the patulin production by P. expansum. The idh gene encodes the isoepoxydon dehydrogenase enzyme, a key enzyme in the patulin biosynthesis pathway. First, a reverse transcription real-time PCR (RT-qPCR) method was optimized to measure accurately the P. expansum idh mRNA levels relative to the mRNA levels of three reference genes (18S, β-tubulin, calmodulin), taking into account important parameters such as PCR inhibition and multiple reference gene stability. Subsequently, two P. expansum field isolates and one reference strain were grown on apple puree agar medium (APAM) under three conditions of temperature and atmosphere: 20°C - air, 4°C - air and 4°C - controlled atmosphere (CA; 3% O2). When P. expansum strains reached a 0.5 and 2.0cm colony diameter, idh expression and patulin concentrations were determined b...Continue Reading

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Citations

Apr 1, 2017·Critical Reviews in Food Science and Nutrition·Joanna TannousOlivier Puel
Mar 25, 2020·Microbial Biotechnology·Youming ShenHaifei Li
May 3, 2021·Toxicon : Official Journal of the International Society on Toxinology·Dipendra Kumar MahatoPradeep Kumar

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