Isoflavones isolated from red clover (Trifolium pratense) inhibit smooth muscle contraction of the isolated rat prostate gland

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
A BrandliS Ventura

Abstract

This study investigated whether red clover contains any bioactive constituents which may affect contractility of rat prostatic smooth muscle in an attempt to determine whether its medicinal use in the treatment of benign prostatic hyperplasia is supported by pharmacological effects. A commercially available red clover extract was chemically fractionated and various isoflavones (genistein, formononetin and biochanin A) were isolated from these fractions and their effects on contractility were examined on preparations of the isolated rat prostate gland. Contractile effects of the isolated fractions were compared with commercially available isoflavones (genistein, formononetin and biochanin A). Pharmacological tools were used to investigate the mechanism of action modifying smooth muscle contraction. Crude red clover extract (Trinovin) inhibited electrical field stimulation induced contractions of the rat prostate across a range of frequencies with an IC(50) of approximately 68 microg/ml. Contractions of the rat prostate elicited by exogenous administration of acetylcholine, noradrenaline or adenosine 5'-triphosphate (ATP) were also inhibited. Chromatographic separation, and final purification by high performance liquid chromatogr...Continue Reading

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Citations

Jul 9, 2013·Indian Journal of Pharmacology·Bhavin A VyasDinesh R Shah
May 31, 2011·Differentiation; Research in Biological Diversity·Tristan M Nicholson, William A Ricke
Oct 29, 2015·Critical Reviews in Food Science and Nutrition·Laura CornaraBruno Burlando
Oct 13, 2016·Journal of Ethnopharmacology·Wararut BuncharoenChatchote Thitaram
Aug 23, 2018·Phytotherapy Research : PTR·Zahra GholamnezhadMohammad Hossein Boskabady
Aug 31, 2019·BioMed Research International·Dongjun JiangXiaomeng Li
Jan 12, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Annabel SpekMartin Hennenberg
Jan 21, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kensuke MitsunariHideki Sakai

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