Isoflurane inhibits neutrophil-endothelium interactions in the coronary circulation: lack of a role for adenosine triphosphate-sensitive potassium channels

Anesthesia and Analgesia
Guochang HuGeorge J Crystal

Abstract

Isoflurane protects myocardium during ischemia-reperfusion via a mechanism involving the adenosine triphosphate-sensitive potassium channels. We tested the hypothesis that an inhibition of the neutrophil-endothelium interactions by isoflurane contributes to this effect. Polymorphonuclear neutrophils and coronary artery segments were obtained from 35 healthy dogs. Superoxide production by neutrophils, stimulated with platelet-activating factor (PAF; 1.0 microM), was measured spectrophotometrically. Adherence of PAF-activated neutrophils to the endothelium of coronary segments was assessed by direct counting of neutrophils labeled with fluorescent dye. Coronary artery rings were exposed to PAF-activated neutrophils, and, after washing and preconstriction with U46619, they were evaluated for vasorelaxation responses to acetylcholine (endothelium dependent) and sodium nitroprusside (endothelium independent). Measurements were performed in the absence and presence of isoflurane (1 and 2 minimum alveolar anesthetic concentration) both with and without glibenclamide (10 microM). Isoflurane inhibited superoxide production and adherence by neutrophils and abolished neutrophil-induced reductions in coronary vascular relaxation responses ...Continue Reading

References

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Citations

Dec 2, 2005·The Laryngoscope·Joung Uk KimJong Woo Chung
Oct 26, 2005·Anesthesia and Analgesia·Guochang HuGeorge J Crystal
Nov 3, 2009·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Maged M SaadGeorge J Crystal
Apr 28, 2004·Anesthesiology·Katsuya TanakaDavid C Warltier
Aug 8, 2008·Journal of Anesthesia·Shin Kurosawa, Masato Kato
Dec 8, 2011·International Journal of Immunopathology and Pharmacology·F CardinaleR Bernardini
Apr 13, 2005·The Journal of Trauma·James W SuliburkDavid W Mercer

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