PMID: 16645452Apr 29, 2006Paper

Isoflurane neuroprotection in rat hippocampal slices decreases with aging: changes in intracellular Ca2+ regulation and N-methyl-D-aspartate receptor-mediated Ca2+ influx

Anesthesiology
Xinhua ZhanPhilip E Bickler

Abstract

Most in vitro neuroprotection studies with isoflurane have involved cells obtained during the embryonic or early postnatal period. However, in mature rodents, isoflurane neuroprotection does not persist. The authors determined whether neuroprotection of hippocampal slices with isoflurane decreases with aging and is due to decreased intracellular Ca regulation and survival protein phosphorylation. Hippocampal slices from 5-day-old, 1-month-old, and 19- to 23-month-old rats were deprived of oxygen and glucose for 5-30 min in media bubbled with 1% isoflurane. Cell death was assessed in the CA1, CA3, and dentate regions, and intracellular Ca concentration was measured in CA1 neurons. N-methyl-d-aspartate receptor (NMDAR)-dependent Ca influx was measured and the phosphorylation of NMDARs, and the survival proteins Akt and mitogen-activated protein kinase p42/44 were quantified. Twenty minutes of oxygen and glucose deprivation killed approximately 40-60% of neurons in CA3 and dentate in all age groups. Isoflurane, 1%, reduced death of CA1, CA3, and dentate neurons in slices from 5-day-old rats but not those from 23-month-old rats. In 5-day slices, isoflurane attenuated NMDAR-mediated Ca influx, whereas in aging slices, Ca influx was ...Continue Reading

References

Jun 1, 1995·Journal of Neuroscience Methods·A SchurrB M Rigor
Jan 1, 1993·Journal of Neurophysiology·E C Burgard, J J Hablitz
Aug 28, 1998·British Journal of Pharmacology·A OuanounouH El-Beheiry
May 21, 1999·The European Journal of Neuroscience·F FerragutiC Corti
Oct 30, 2001·Anesthesia and Analgesia·L Wise-FaberowskiC Sumners
Dec 26, 2001·Anesthesiology·Breandan L SullivanPhilip E Bickler
Jun 27, 2003·Journal of Neurosurgical Anesthesiology·L Wise-FaberowskiC Sumners
Feb 26, 2004·Anesthesia and Analgesia·Masahiko KawaguchiPiyush M Patel
Mar 23, 2004·Current Molecular Medicine·Chainllie YoungJohn W Olney
Aug 7, 2004·Brain Research. Brain Research Protocols·Getaw W HassenPeter J Bergold
Sep 18, 2004·Trends in Neurosciences·Emil C ToescuPhilip W Landfield
Feb 19, 2005·Neurobiology of Aging·Vesna Jevtovic-Todorovic, Lisa B Carter
May 6, 2005·Journal of Anesthesia·Masahiko KawaguchiPiyush M Patel
Aug 24, 2005·Anesthesia and Analgesia·Lisa Wise-FaberowskiDavid S Warner

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Citations

Apr 30, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jiang ZhuLixin Liu
Jun 6, 2009·European Journal of Anaesthesiology·Andrea Kopp LugliChristoph H Kindler
May 24, 2008·Anesthesia and Analgesia·Andreas W Loepke, Sulpicio G Soriano
May 24, 2008·Anesthesia and Analgesia·Andreas W LoepkeSulpicio G Soriano
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Guohua ZhangZhongcong Xie
Sep 9, 2006·Current Opinion in Anaesthesiology·Ines P Koerner, Ansgar M Brambrink
Aug 21, 2012·Anesthesiology Clinics·Ansgar M BrambrinkJeffrey R Kirsch
Aug 26, 2009·Anesthesiology Clinics·Jimmy W Huh, Ramesh Raghupathi
Aug 26, 2009·Anesthesiology Clinics·Mary Ellen McCann, Sulpicio G Soriano
May 23, 2008·Best Practice & Research. Clinical Anaesthesiology·Jane Sturgess, Basil Matta
Nov 27, 2008·Acta Anaesthesiologica Scandinavica·Ravi BainsJ Berg-Johnsen
Apr 10, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sherri L ChristianKelly L Drew
Dec 14, 2019·Anesthesiology·Roderic G EckenhoffMaryellen F Eckenhoff

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