Mar 5, 2020

Isoform cell type specificity in the mouse primary motor cortex

BioRxiv : the Preprint Server for Biology
A. Sina BooeshaghiLior Pachter

Abstract

Full-length SMART-Seq single-cell RNA-seq can be used to measure gene expression at isoform resolution, making possible the identification of isoform markers for cell types and for an isoform atlas. In a comprehensive analysis of 6,160 mouse primary motor cortex cells assayed with SMART-Seq, we find numerous examples of isoform specificity in cell types, including isoform shifts between cell types that are masked in gene-level analysis. These findings can be used to refine spatial gene expression information to isoform resolution. Our results highlight the utility of full-length single-cell RNA-seq when used in conjunction with other single-cell RNA-seq technologies.

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Mentioned in this Paper

Protein Isoforms
Gene Expression Analysis
Cell Type
Genes
Gene Expression
Pyramidal Cell of Precentral Gyrus of Cerebral Hemisphere
Finding
Cell Separation
Analysis
Laboratory mice

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