Isoforms of cyclic AMP response element binding proteins in Drosophila S2 cells

Biochemical and Biophysical Research Communications
J PoelsJ Vanden Broeck

Abstract

Activation or inhibition of the cyclic AMP (cAMP)-protein kinase A (PKA) pathway can ultimately regulate the transcription of a variety of genes. In vertebrates, the best characterized nuclear targets of PKA are the 'cAMP response element' (CRE) binding proteins (CREB). Differences in the transcriptional response to this pathway between cells and tissues can be based on the presence of distinct CREB isoforms. In this context, we have now investigated the presence of different dCREB transcripts in a stable, embryonic insect cell line, i.e., Drosophila Schneider 2 (S2) cells. In addition, we have studied the possible effect of cellular cAMP- and Ca2+ increases on the expression of a luciferase reporter in cells transfected with a CRE-containing reporter gene construct. In combination with recent data from the literature, our results indicate that the regulation of CRE-dependent gene expression shows some important differences between insects and vertebrates.

References

Oct 1, 1992·Trends in Biochemical Sciences·M Karin, T Smeal
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M R MontminyR H Goodman
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·K FerreriM Montminy
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·E E Saffman, M A Krasnow
Sep 1, 1993·DNA and Cell Biology·T UsuiR H Goodman
Oct 28, 1993·Nature·J C ChriviaR H Goodman
Jan 1, 1995·Annual Review of Cell and Developmental Biology·P Sassone-Corsi
May 7, 1999·Neuron·M P BelvinJ C Yin
Jun 16, 2000·Molecular and Cellular Endocrinology·W J Roesler
Jul 31, 2001·Molecular and Cellular Endocrinology·H L WilsonW J Roesler
Mar 26, 2002·Molecular and Cellular Endocrinology·Heather L Wilson, William J Roesler
Sep 17, 2002·EMBO Reports·Yoshishige KimuraHiroshi Tokumitsu
Dec 3, 2002·Journal of Molecular Endocrinology·C VyhlidalS Safe
Dec 7, 2002·Behavior Genetics·Takaomi Sakai, Yoshiaki Kidokoro
Dec 4, 2003·Gene·Birgit E J TeunissenMarti F A Bierhuizen

❮ Previous
Next ❯

Citations

Apr 27, 2020·Archives of Insect Biochemistry and Physiology·Umut ToprakGözde Güney

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.