PMID: 9790304Oct 28, 1998

Isoforms of murine and human serum amyloid P component

Scandinavian Journal of Immunology
M NyboS E Svehag

Abstract

Isoelectric focusing (IEF) and immunofixation of murine serum amyloid P component (SAP), purified and in serum, showed a distinct and strain-dependent isoform pattern with up to seven bands (pI 5.1-5.7). Neuraminidase treatment caused a shift of the isoforms to more basic pI values, but did not affect their number. When the acute-phase response was analysed in three mouse strains, CBA/J and C3H/HeN initially showed seven SAP isoforms in serum and C57BL/6 J three or four. The responses in all three strains peaked at day 2 and were normalized within 14 days. On days 2 and 4, CBA/J and C3H/HeN mice showed one more acidic isoform and an increase in the concentration of the most basic isoform. C57BL/6 J mice exhibited two to three new isoforms during the acute-phase response. This appears to be the first demonstration of the physiological existence of SAP isoforms. In contrast, demonstration of isoforms of human SAP required the presence of urea and higher SAP concentrations. TEF and immunofixation of SAP monomers showed five to eight isoforms, ranging from pI 4.7-5.7. IEF of SAP in human serum resulted in a less distinct pattern and more acidic isoforms. As with murine SAP, neuraminidase treatment caused a shift of the isoforms, bu...Continue Reading

References

Jan 17, 2012·Proteomics·E GianazzaIngrid Miller
Dec 28, 1999·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·M NyboS E Svehag

Citations

May 1, 1991·Clinical and Experimental Immunology·P N HawkinsM B Pepys
Jan 1, 1989·Acta Neuropathologica·T DuongA B Scheibel
Jan 1, 1988·International Archives of Allergy and Applied Immunology·B M KubakL A Potempa
Oct 23, 1987·Journal of Immunological Methods·H Haas, M Schlaak
Oct 14, 1987·Biochemical and Biophysical Research Communications·M B Pepys, P J Butler
May 30, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·I OhkuboM Sasaki
Jan 1, 1982·Annals of the New York Academy of Sciences·M B PepysA Feinstein
Jan 1, 1982·Annals of the New York Academy of Sciences·M L BaltzM B Pepys
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Jan 1, 1995·International Archives of Allergy and Immunology·I J SørensenS E Svehag
Jan 27, 1994·Nature·J EmsleyS P Wood
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·M B PepysJ Herbert
Jun 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C G DrakeB L Kotzin
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Mar 1, 1997·Archives of Biochemistry and Biophysics·I ParmrydG Dallner

Related Concepts

Mice, Inbred CBA
Acute-Phase Reaction
Neu1
C57BL/6 Mouse
Murine
Properdin Deficiency, X-LINKED
CBA/J Mouse
Mice, Inbred C3H
Neuraminidase
C3H/HeN Mouse

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