Isoforms of rat liver fatty acid binding protein differ in structure and affinity for fatty acids and fatty acyl CoAs

Biochemistry
A FrolovF Schroeder

Abstract

Although native rat liver fatty acid binding protein (L-FABP) is composed of isoforms differing in isoelectric point, their comparative structure and function are unknown. These properties of apo- and holo-L-FABP isoforms were resolved by circular dichroism, time-resolved fluorescence spectroscopy, and binding/displacement of fluorescent ligands. Both apo-isoforms had similar hydrodynamic radii of 18.5 A, but apo-isoform I had a greater alpha-helical content and exhibited a longer Tyr lifetime, indicative of secondary and tertiary structural differences from isoform II. Isoforms I and II both had two fatty acid or fatty acyl CoA binding sites. Ligand binding decreased the isoform hydrodynamic radii by 3-4 A and increased Tyr rotational motions in a more restricted range. Fatty acyl CoAs were more effective than fatty acids in altering the isoform structures. Scatchard analysis showed that both isoforms bound cis- parinaric acid with high affinity (Kd values 41 and 60 nM, respectively) and bound trans-parinaric acid with 2- and 7-fold, respectively, higher affinity than for cis-parinaric acid. In contrast, isoform I had higher affinity for cis- and trans-parinaroyl CoAs (Kd values of 33 and 14 nM) than did isoform II (Kd values ...Continue Reading

References

Nov 1, 1992·Biochemical Society Transactions·I IssemannS Green
Apr 1, 1991·Archives of Biochemistry and Biophysics·G NemeczF Schroeder
Nov 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S D Clarke, M K Armstrong
Jan 1, 1989·Progress in Lipid Research·V MatareseD A Bernlohr
Sep 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J P PignonC S Lieber
Jan 4, 1988·European Journal of Biochemistry·H Schulenberg-SchellF Spener
Jun 23, 1987·Biochimica Et Biophysica Acta·R E BurrierP Brecher
Aug 30, 1985·Chemistry and Physics of Lipids·N M Bass
Jul 1, 1983·Analytical Biochemistry·J F Glatz, J H Veerkamp
Jul 1, 1981·Archives of Biochemistry and Biophysics·D Trulzsch, I M Arias
Mar 1, 1995·The Proceedings of the Nutrition Society·J H Veerkamp
Jan 1, 1995·Progress in Lipid Research·J H Veerkamp, R G Maatman
Dec 1, 1994·Cancer Metastasis Reviews·S Sorof
Dec 30, 1994·Biochemical and Biophysical Research Communications·P B BurtonM J Tynan
Dec 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J H VeerkampC F Prinsen
Jan 1, 1994·Advances in Protein Chemistry·L BanaszakT A Jones
Dec 8, 1993·Molecular and Cellular Biochemistry·G SaM Mukherjea
Jun 9, 1993·Molecular and Cellular Biochemistry·K R Miller, D P Cistola
Jun 9, 1993·Molecular and Cellular Biochemistry·F SchroederD Moncecchi
Jul 21, 1993·Biochimica Et Biophysica Acta·A K Dutta-RoyP Trayhurn
Aug 1, 1993·European Journal of Biochemistry·N BartetzkoF Spener
Jan 1, 1996·Journal of Neurochemistry·S C Myers-PayneF Schroeder
Apr 1, 1996·Journal of Neurochemistry·S C Myers-PayneF Schroeder

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Citations

May 5, 2011·Molecular and Cellular Biochemistry·Heather A HostetlerFriedhelm Schroeder
Jun 19, 1998·Chemistry and Physics of Lipids·F SchroederA Frolov
Jan 30, 2002·Progress in Lipid Research·Simon Eaton
May 29, 2000·Biochimica Et Biophysica Acta·S M Di Pietro, J A Santomé
Jun 15, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·S MeinersH M Geller
Feb 10, 2000·Biochimica Et Biophysica Acta·D H AlpersK DeSchryver-Kecskemeti
Oct 10, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gregory G MartinFriedhelm Schroeder
Aug 4, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stephen M StoreyFriedhelm Schroeder
Aug 2, 2008·BMC Genomics·Massimo Bionaz, Juan J Loor
Apr 21, 2011·Human Genomics·Rebecca L Smathers, Dennis R Petersen
Mar 18, 2009·Journal of Lipid Research·Heather A HostetlerFriedhelm Schroeder
Mar 21, 1998·Archives of Biochemistry and Biophysics·R E GossettF Schroeder
Jun 12, 2013·Biochimica Et Biophysica Acta·Anca D PetrescuFriedhelm Schroeder
Sep 25, 2014·International Journal of Molecular Sciences·Nan LiXue-Jun Gao
Jun 12, 2010·The Journal of Nutritional Biochemistry·Barbara P AtshavesFriedhelm Schroeder
Jun 5, 2007·Biochimica Et Biophysica Acta·Friedhelm SchroederAnn B Kier
Jun 28, 2015·Archives of Biochemistry and Biophysics·Gregory G MartinAnn B Kier
Mar 4, 2015·Biochimica Et Biophysica Acta·Huan HuangFriedhelm Schroeder
May 12, 2016·Journal of Neurochemistry·Gregory G MartinFriedhelm Schroeder
Aug 24, 2016·Biochemistry·Huan HuangFriedhelm Schroeder
Nov 20, 2016·Journal of Neurochemistry·Gregory G MartinFriedhelm Schroeder
Apr 16, 2017·Journal of Lipid Research·Stephen M StoreyFriedhelm Schroeder
Dec 10, 1999·The Journal of Biological Chemistry·N StolowichF Schroeder
Oct 4, 2017·Journal of Lipid Research·Gregory G MartinFriedhelm Schroeder
May 25, 2004·The Journal of Biological Chemistry·Barbara P AtshavesFriedhelm Schroeder

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